mineralmedium_with_6_methylnicotinate

CultureMech:001593 bacterial COMPLEX SOLID_AGAR
Original name: MINERALMEDIUM WITH 6-METHYLNICOTINATE

Overview

External ontology
mediadive.medium:465h (source link unavailable) — MINERALMEDIUM WITH 6-METHYLNICOTINATE
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465h.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001593["mineralmedium_with_6_methylnicotinate"]
    ing0_6_methylnicotinate["6-methylnicotinate"]
    CultureMech_001593 --> ing0_6_methylnicotinate
    ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001593 --> ing1_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001593 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001593 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001593 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_001593 --> ing5_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing5_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing6_NaOH["NaOH"]
    CultureMech_001593 --> ing6_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing6_NaOH -.-> CHEBI_32145
    ing7_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001593 --> ing7_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing7_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing8_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001593 --> ing8_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing8_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing9_Vitamin_B12["Vitamin B12"]
    CultureMech_001593 --> ing9_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing9_Vitamin_B12 -.-> CHEBI_176843
    ing10_Pantothenic_acid["Pantothenic acid"]
    CultureMech_001593 --> ing10_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing10_Pantothenic_acid -.-> CHEBI_7916
    ing11_Riboflavin["Riboflavin"]
    CultureMech_001593 --> ing11_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing11_Riboflavin -.-> CHEBI_17015
    ing12_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_001593 --> ing12_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing12_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing13_Biotin["Biotin"]
    CultureMech_001593 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Folic_acid["Folic acid"]
    CultureMech_001593 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001593 --> ing15_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing15_Nicotinic_acid -.-> CHEBI_15940
    ing16_Nicotine_amide["Nicotine amide"]
    CultureMech_001593 --> ing16_Nicotine_amide
    CHEBI_17154["CHEBI:17154"]
    ing16_Nicotine_amide -.-> CHEBI_17154
    ing17_alpha_lipoic_acid["alpha-lipoic acid"]
    CultureMech_001593 --> ing17_alpha_lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing17_alpha_lipoic_acid -.-> CHEBI_16494
    ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001593 --> ing18_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing19_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001593 --> ing19_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing19_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing20_Na2_EDTA["Na2-EDTA"]
    CultureMech_001593 --> ing20_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing20_Na2_EDTA -.-> CHEBI_64734
    ing21_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001593 --> ing21_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing21_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001593 --> ing22_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing23_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001593 --> ing23_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing23_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing24_H3BO3["H3BO3"]
    CultureMech_001593 --> ing24_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing24_H3BO3 -.-> CHEBI_33118
    ing25_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001593 --> ing25_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing25_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing26_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001593 --> ing26_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing26_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing27_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001593 --> ing27_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing27_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001593 --> ing28_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing29_Peptone["Peptone"]
    CultureMech_001593 --> ing29_Peptone
    CultureMech_001593 --> more["...19 more"]

Ingredients (49)

# Ingredient Concentration Ontology Notes
1 6-methylnicotinate 5 G_PER_L (adjust if required)
2 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
3.5 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
3 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.05 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
7 NaOH
HO.Na 39.997 g/mol
500 MG_PER_L CHEBI:32145
sodium hydroxide
8 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
3 MG_PER_L CHEBI:131361
disodium selenite pentahydrate
9 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
4 MG_PER_L CHEBI:63939
sodium tungstate dihydrate
10 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
11 Pantothenic acid
C9H17NO5 219.237 g/mol
0.05 G_PER_L CHEBI:7916
pantothenic acid
12 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
13 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.01 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
14 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
15 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
16 Nicotinic acid
C6H5NO2 123.111 g/mol
0.025 G_PER_L CHEBI:15940
nicotinic acid
17 Nicotine amide
C6H6N2O 122.127 g/mol
0.025 G_PER_L CHEBI:17154
nicotinamide
18 alpha-lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
19 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
20 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.05 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
21 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
22 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
23 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
24 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
25 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
26 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
27 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
28 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
29 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
30 Peptone 5 G_PER_L
31 Meat extract 3 G_PER_L
32 Agar 65.0 G_PER_L CHEBI:2509
agar
 (for solid medium) [Merged 4 duplicates: 15.0, 15.0, 20.0, 15.0]
33 Casein peptone 15 G_PER_L
34 Soy peptone 5 G_PER_L
35 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
36 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
37 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
38 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
39 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
40 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
41 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
42 Yeast extract 0.5 G_PER_L
43 Proteose peptone 0.5 G_PER_L
44 Casamino acids 0.5 G_PER_L
45 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
46 Starch 0.5 G_PER_L CHEBI:28017
starch
47 Na-pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
48 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
49 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate

Preparation

  1. Supplement medium 465 with selenite, tungstate, 2.5 - 5 g/l 6-methylnicotinate from a stock solution (autoclave separately) and 1 ml/l vitamin solution (see medium 461).
  2. pH 7.25
  3. Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.
  4. Autoclave separately. Add 1 ml per l medium.
  5. Stir for some hours, filter sterilize the solution.
  6. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
  7. Adjust pH to 7.0.
  8. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
  9. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
  10. pH 7.3
  11. Autoclave at 121°C for 15 min.
  12. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.261079Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 465h
2026-01-28T19:48:24.453682Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:10.398584Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:05.497700Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001593
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.624544+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERALMEDIUM WITH 6-METHYLNICOTINATE" to "mineralmedium_with_6_methylnicotinate" for consistent matching
2026-06-05T07:41:34.599338+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:48.465826+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:47:08.047526+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.