Overview
- External ontology
- mediadive.medium:467 (source link unavailable) — OTTOW MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium467.pdf
Composition
flowchart LR
CultureMech_001596["ottow_medium"]
ing0_Glucose["Glucose"]
CultureMech_001596 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Peptone["Peptone"]
CultureMech_001596 --> ing1_Peptone
ing2_Meat_extract["Meat extract"]
CultureMech_001596 --> ing2_Meat_extract
ing3_Yeast_extract["Yeast extract"]
CultureMech_001596 --> ing3_Yeast_extract
ing4_Casamino_acids["Casamino acids"]
CultureMech_001596 --> ing4_Casamino_acids
ing5_NaCl["NaCl"]
CultureMech_001596 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Tap_water["Tap water"]
CultureMech_001596 --> ing6_Tap_water
CHEBI_15377["CHEBI:15377"]
ing6_Tap_water -.-> CHEBI_15377
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Peptone | 7.5 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
|
| 3 | Meat extract | 5 G_PER_L | ||
| 4 | Yeast extract | 2.5 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 5 | Casamino acids | 2.5 G_PER_L |
FOODON:03315719
Casamino acids
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
Tap water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
1000 ml tap-water input per recipe batch; not a mass concentration, percentage, or measured final volume. |
Preparation
- Adjust pH to 8.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.273290Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 467 |
| 2026-01-28T19:48:27.461563Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:05.526216Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001596 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.655941+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "OTTOW MEDIUM" to "ottow_medium" for consistent matching |
| 2026-06-05T07:41:40.670946+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.525336+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-09-11T00:00:00-07:00 | repair_togo_m2316_score15.py | LINKED_TOGO_M2316_SOURCE_DUPLICATE | Linked TOGO M2316 as a source duplicate of DSMZ Medium 467. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m3060_score15.py | LINKED_TOGO_M3060_SUPPLEMENTED_VARIANT | Linked TOGO M3060 as an NBRC supplemented variant of DSMZ Medium 467. |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch7.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH7 | Applied local mappings for Casamino acids, Peptone, Yeast extract. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |
| 2026-10-08T00:32:53.463093+00:00 | codex | CORRECTED_DSMZ467_WATER_AND_DSMZ469_FILTERED_PREPARATIONS | Corrected source water input units; for medium 469 restored separate filtered preparations and withdrew unsupported stock-rate interpretations. Preserved earlier history, snippets, mappings and variant links. API/PDF preparation-detail difference remains explicit; no growth association added. |