Overview
- External ontology
- mediadive.medium:480d (source link unavailable) — THERMOPROTEUS UZONIENSIS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.7, 'max': 6.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium480d.pdf
Composition
flowchart LR
CultureMech_001609["thermoproteus_uzoniensis_medium"]
ing0_Casitone["Casitone"]
CultureMech_001609 --> ing0_Casitone
ing1_Biotin["Biotin"]
CultureMech_001609 --> ing1_Biotin
CHEBI_15956["CHEBI:15956"]
ing1_Biotin -.-> CHEBI_15956
ing2_Folic_acid["Folic acid"]
CultureMech_001609 --> ing2_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing2_Folic_acid -.-> CHEBI_27470
ing3_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001609 --> ing3_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing3_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing4_Thiamine_HCl["Thiamine HCl"]
CultureMech_001609 --> ing4_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing4_Thiamine_HCl -.-> CHEBI_49105
ing5_Riboflavin["Riboflavin"]
CultureMech_001609 --> ing5_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing5_Riboflavin -.-> CHEBI_17015
ing6_Nicotinic_acid["Nicotinic acid"]
CultureMech_001609 --> ing6_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing6_Nicotinic_acid -.-> CHEBI_15940
ing7_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001609 --> ing7_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing7_Calcium_D_____pantothenate -.-> CHEBI_31345
ing8_Vitamin_B12["Vitamin B12"]
CultureMech_001609 --> ing8_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing8_Vitamin_B12 -.-> CHEBI_176843
ing9_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001609 --> ing9_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing9_p_Aminobenzoic_acid -.-> CHEBI_30753
ing10__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001609 --> ing10__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing10__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing11_HCl["HCl"]
CultureMech_001609 --> ing11_HCl
CHEBI_17883["CHEBI:17883"]
ing11_HCl -.-> CHEBI_17883
ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001609 --> ing12_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
ing13_ZnCl2["ZnCl2"]
CultureMech_001609 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001609 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_H3BO3["H3BO3"]
CultureMech_001609 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001609 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001609 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001609 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001609 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_NH4Cl["NH4Cl"]
CultureMech_001609 --> ing20_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing20_NH4Cl -.-> CHEBI_31206
ing21_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001609 --> ing21_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing21_CaCl2_x_2_H2O -.-> CHEBI_86158
ing22_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001609 --> ing22_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing22_MgCl2_x_6_H2O -.-> CHEBI_86345
ing23_KCl["KCl"]
CultureMech_001609 --> ing23_KCl
CHEBI_32588["CHEBI:32588"]
ing23_KCl -.-> CHEBI_32588
ing24_KH2PO4["KH2PO4"]
CultureMech_001609 --> ing24_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing24_KH2PO4 -.-> CHEBI_63036
ing25_Yeast_extract["Yeast extract"]
CultureMech_001609 --> ing25_Yeast_extract
ing26_Sodium_resazurin["Sodium resazurin"]
CultureMech_001609 --> ing26_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing26_Sodium_resazurin -.-> CHEBI_8806
ing27_Sulfur["Sulfur"]
CultureMech_001609 --> ing27_Sulfur
CHEBI_26833["CHEBI:26833"]
ing27_Sulfur -.-> CHEBI_26833
ing28_Na_acetate["Na-acetate"]
CultureMech_001609 --> ing28_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing28_Na_acetate -.-> CHEBI_32954
ing29_NaHCO3["NaHCO3"]
CultureMech_001609 --> ing29_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing29_NaHCO3 -.-> CHEBI_32139
CultureMech_001609 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casitone | 3 G_PER_L | ||
| 2 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 3 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 4 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 5 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 6 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 7 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 8 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 9 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 10 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 11 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 12 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 13 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 22 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.33 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 23 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.33 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 24 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 25 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 26 | Yeast extract | 0.1 G_PER_L | ||
| 27 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 28 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 29 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 30 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 31 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Use medium 480, but reduce the amount of Na acetate to 1.0 g/l and add 3.0 g/l casitone. Prepare sulphide and vitamin stock solutions anaerobically. Autoclave the sulphide solution but filter sterilise the vitamins. Prepare the medium anaerobically under N2:CO2 80:20. Adjust the pH to 5.7 with HCl. Sterilise the medium by heating for 3 hours on three successive days at 90-100°C. Add the vitamins and sulphide to the sterilised medium before use. The final pH should be 5.7-6.0. Dr. Bonch-Osmoloskaya supplied the information that the strain grows better on a peptone originally available in Russia. This product is no longer available, but it appears this product gave better growth than with highly purified products from Oxoid or Difco.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
- Dissolve ingredients (except sulfur, acetate, bicarbonate, vitamins and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 5.9, dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and sterilize by heating for 2-3 hours in a boiling water bath on each of 3 successive days. Add acetate, vitamins (sterilized by filtration)and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.321458Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 480d |
| 2026-02-06T05:10:23.145611Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:05.655511Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001609 |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.794672+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOPROTEUS UZONIENSIS MEDIUM" to "thermoproteus_uzoniensis_medium" for consistent matching |
| 2026-05-20T06:02:08.563786+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5.7-6' -> {'min': 5.7, 'max': 6.0} |
| 2026-06-05T07:41:50.500970+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:13.147537+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |