Overview
- External ontology
- mediadive.medium:492 (source link unavailable) — CLOSTRIDIUM METHYLPENTOSUM MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium492.pdf
Composition
flowchart LR
CultureMech_001620["clostridium_methylpentosum_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001620 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_001620 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NaCl["NaCl"]
CultureMech_001620 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001620 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001620 --> ing4_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001620 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001620 --> ing6_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001620 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001620 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_CuSO4_x_H2O["CuSO4 x H2O"]
CultureMech_001620 --> ing9_CuSO4_x_H2O
CHEBI_23414["CHEBI:23414"]
ing9_CuSO4_x_H2O -.-> CHEBI_23414
ing10_Sodium_resazurin["Sodium resazurin"]
CultureMech_001620 --> ing10_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Sodium_resazurin -.-> CHEBI_8806
ing11_NaHCO3["NaHCO3"]
CultureMech_001620 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_L_Rhamnose["L-Rhamnose"]
CultureMech_001620 --> ing12_L_Rhamnose
CHEBI_62345["CHEBI:62345"]
ing12_L_Rhamnose -.-> CHEBI_62345
ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001620 --> ing13_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing14_Biotin["Biotin"]
CultureMech_001620 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Folic_acid["Folic acid"]
CultureMech_001620 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001620 --> ing16_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing17_Thiamine_HCl["Thiamine HCl"]
CultureMech_001620 --> ing17_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing17_Thiamine_HCl -.-> CHEBI_49105
ing18_Riboflavin["Riboflavin"]
CultureMech_001620 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_001620 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001620 --> ing20_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_D_____pantothenate -.-> CHEBI_31345
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_001620 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001620 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001620 --> ing23__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing23__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.943396 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.943396 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.943396 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0209644 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0209644 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0209644 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.00524109 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00524109 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00209644 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
CuSO4 x H2O
Cu.O4S 159.609 g/mol
|
0.00209644 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
|
| 11 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000524109 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
50 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 | L-Rhamnose | 200 G_PER_L |
CHEBI:62345
L-rhamnose
|
|
| 14 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
50 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 18 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Sparge solution A with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions C and D are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution E is autoclaved separately under 100% N2 gas. Solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 6.8 - 7.0.
- Adjust pH to 6.5 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.359459Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 492 |
| 2026-02-06T05:10:20.635497Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:05.783066Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001620 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.929631+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM METHYLPENTOSUM MEDIUM" to "clostridium_methylpentosum_medium" for consistent matching |
| 2026-05-20T06:01:11.967352+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0} |
| 2026-06-05T07:40:54.085950+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.756337+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |