pseudothermotoga_medium

CultureMech:001625 bacterial COMPLEX LIQUID
Original name: PSEUDOTHERMOTOGA MEDIUM

Overview

External ontology
mediadive.medium:498 (source link unavailable) — PSEUDOTHERMOTOGA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium498.pdf

Composition

flowchart LR
    CultureMech_001625["pseudothermotoga_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001625 --> ing0_Yeast_extract
    ing1_NaCl["NaCl"]
    CultureMech_001625 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Na2_EDTA["Na2-EDTA"]
    CultureMech_001625 --> ing2_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing2_Na2_EDTA -.-> CHEBI_64734
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001625 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001625 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001625 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KCl["KCl"]
    CultureMech_001625 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaBr["NaBr"]
    CultureMech_001625 --> ing7_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing7_NaBr -.-> CHEBI_63004
    ing8_H3BO3["H3BO3"]
    CultureMech_001625 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9__NH4_2Ni_SO4_2["(NH4)2Ni(SO4)2"]
    CultureMech_001625 --> ing9__NH4_2Ni_SO4_2
    CHEBI_86149["CHEBI:86149"]
    ing9__NH4_2Ni_SO4_2 -.-> CHEBI_86149
    ing10_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001625 --> ing10_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing10_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing11_KI["KI"]
    CultureMech_001625 --> ing11_KI
    CHEBI_8346["CHEBI:8346"]
    ing11_KI -.-> CHEBI_8346
    ing12_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001625 --> ing12_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Sodium_resazurin -.-> CHEBI_8806
    ing13_KH2PO4["KH2PO4"]
    CultureMech_001625 --> ing13_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing13_KH2PO4 -.-> CHEBI_63036
    ing14_Starch["Starch"]
    CultureMech_001625 --> ing14_Starch
    CHEBI_28017["CHEBI:28017"]
    ing14_Starch -.-> CHEBI_28017
    ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001625 --> ing15_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_x_9_H2O -.-> CHEBI_76209
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001625 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001625 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001625 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001625 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001625 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001625 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001625 --> ing22_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001625 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001625 --> ing24_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001625 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001625 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.488281 G_PER_L
2 NaCl
Cl.Na 58.443 g/mol
4.378909999999999 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 3.37891, 1.0]
3 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.751953 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.859375 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.859375, 3.0]
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.673828 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.236719 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.136719, 0.1]
7 KCl
Cl.K 74.551 g/mol
0.078125 G_PER_L CHEBI:32588
potassium chloride
8 NaBr
Br.Na 102.894 g/mol
0.012207 G_PER_L CHEBI:63004
sodium bromide
9 H3BO3
H3BO3 61.833 g/mol
0.01390625 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.00390625, 0.01]
10 (NH4)2Ni(SO4)2
6H2O.2H4N.Ni.2O4S 394.987 g/mol
0.00292969 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
11 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.00185547 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
12 KI
I.K 166.002 g/mol
9.76562e-06 G_PER_L CHEBI:8346
potassium iodide
13 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000488281 G_PER_L CHEBI:8806
Resazurin
14 KH2PO4
H2O4P.K 136.084 g/mol
0.488281 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
15 Starch 4.88281 G_PER_L CHEBI:28017
starch
16 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.488281 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
23 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
26 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
27 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients (except hydrogenphosphate, starch and sulfide) and adjust pH to 7.0. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% volume and autoclave. Add hydrogenphosphate, starch and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Check pH of the complete medium and adjust to 7.0 - 7.2, if necessary.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.380298Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 498
2026-03-13T03:09:38.757338Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:05.858079Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001625
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.007807+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PSEUDOTHERMOTOGA MEDIUM" to "pseudothermotoga_medium" for consistent matching
2026-05-20T06:02:03.146828+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:41:42.808946+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.502597+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.