carboxydothermus_medium_heterotroph

Overview

External ontology

mediadive.medium:508 (source link unavailable) — CARBOXYDOTHERMUS MEDIUM (HETEROTROPH)

Applications

• Microbial cultivation

pH range

{'min': 6.8, 'max': 7.0}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium508.pdf

Composition

flowchart LR
    CultureMech_001642["carboxydothermus_medium_heterotroph"]
    ing0_KCl["KCl"]
    CultureMech_001642 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001642 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001642 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001642 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KH2PO4["KH2PO4"]
    CultureMech_001642 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001642 --> ing5_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Sodium_resazurin -.-> CHEBI_8806
    ing6_Na2CO3["Na2CO3"]
    CultureMech_001642 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_Na_pyruvate["Na-pyruvate"]
    CultureMech_001642 --> ing7_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing7_Na_pyruvate -.-> CHEBI_50144
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_001642 --> ing8_Yeast_extract
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001642 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Na2_EDTA["Na2-EDTA"]
    CultureMech_001642 --> ing10_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing10_Na2_EDTA -.-> CHEBI_64734
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001642 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001642 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001642 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_001642 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001642 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001642 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001642 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001642 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_D_____biotin["D-(+)-biotin"]
    CultureMech_001642 --> ing19_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_D_____biotin -.-> CHEBI_15956
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001642 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345
    ing21_Biotin["Biotin"]
    CultureMech_001642 --> ing21_Biotin
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_001642 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001642 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_001642 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001642 --> ing25_Calcium_D_____pantothenate
    ing25_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing26__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_001642 --> ing26__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26__DL__alpha_Lipoic_acid -.-> CHEBI_16494

Ingredients (27)

Preparation

1. Dissolve ingredients except carbonate, pyruvate, yeast extract, vitamins and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 -45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate, yeast extract, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of pyruvate and vitamins are sterilized by filtration. After completing the medium adjust pH to 6.8-7.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v), if necessary.
2. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Solutions

Target organisms

No target organisms are recorded.

Provenance