reactivation_with_liquid_medium_514

CultureMech:001647 bacterial COMPLEX SOLID_AGAR
Original name: REACTIVATION WITH LIQUID MEDIUM 514

Overview

External ontology
mediadive.medium:514c (source link unavailable) — REACTIVATION WITH LIQUID MEDIUM 514
Applications
  • Microbial cultivation
pH
7.6
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514c.pdf

Composition

flowchart LR
    CultureMech_001647["reactivation_with_liquid_medium_514"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_001647 --> ing0_Bacto_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001647 --> ing1_Yeast_extract
    ing2_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_001647 --> ing2_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing2_Fe_III__citrate -.-> CHEBI_144421
    ing3_NaCl["NaCl"]
    CultureMech_001647 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2["MgCl2"]
    CultureMech_001647 --> ing4_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing4_MgCl2 -.-> CHEBI_6636
    ing5_Na2SO4["Na2SO4"]
    CultureMech_001647 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_CaCl2["CaCl2"]
    CultureMech_001647 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_KCl["KCl"]
    CultureMech_001647 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_Na2CO3["Na2CO3"]
    CultureMech_001647 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_KBr["KBr"]
    CultureMech_001647 --> ing9_KBr
    CHEBI_32030["CHEBI:32030"]
    ing9_KBr -.-> CHEBI_32030
    ing10_SrCl2["SrCl2"]
    CultureMech_001647 --> ing10_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing10_SrCl2 -.-> CHEBI_36383
    ing11_H3BO3["H3BO3"]
    CultureMech_001647 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_Na_silicate["Na-silicate"]
    CultureMech_001647 --> ing12_Na_silicate
    CHEBI_60720["CHEBI:60720"]
    ing12_Na_silicate -.-> CHEBI_60720
    ing13_NaF["NaF"]
    CultureMech_001647 --> ing13_NaF
    CHEBI_28741["CHEBI:28741"]
    ing13_NaF -.-> CHEBI_28741
    ing14__NH4_NO3["(NH4)NO3"]
    CultureMech_001647 --> ing14__NH4_NO3
    CHEBI_63038["CHEBI:63038"]
    ing14__NH4_NO3 -.-> CHEBI_63038
    ing15_Na2HPO4["Na2HPO4"]
    CultureMech_001647 --> ing15_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing15_Na2HPO4 -.-> CHEBI_34683
    ing16_Agar["Agar"]
    CultureMech_001647 --> ing16_Agar
    CHEBI_2509["CHEBI:2509"]
    ing16_Agar -.-> CHEBI_2509

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Bacto peptone 5 G_PER_L
2 Yeast extract 1 G_PER_L
3 Fe(III) citrate
C6H5FeO7 244.944 g/mol
0.1 G_PER_L CHEBI:144421
iron(III) citrate
4 NaCl
Cl.Na 58.443 g/mol
19.45 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2
2Cl.Mg 95.211 g/mol
5.9 G_PER_L CHEBI:6636
magnesium dichloride
6 Na2SO4
2Na.O4S 142.043 g/mol
3.24 G_PER_L CHEBI:32149
sodium sulfate
7 CaCl2
Ca.2Cl 110.984 g/mol
1.8 G_PER_L CHEBI:3312
calcium dichloride
8 KCl
Cl.K 74.551 g/mol
0.55 G_PER_L CHEBI:32588
potassium chloride
9 Na2CO3
CO3.2Na 105.988 g/mol
0.16 G_PER_L CHEBI:29377
sodium carbonate
10 KBr
Br.K 119.002 g/mol
0.08 G_PER_L CHEBI:32030
potassium bromide
11 SrCl2
2Cl.Sr 158.526 g/mol
0.034 G_PER_L CHEBI:36383
strontium dichloride
12 H3BO3
H3BO3 61.833 g/mol
0.022 G_PER_L CHEBI:33118
boric acid
13 Na-silicate
2Na.O3Si 122.063 g/mol
0.004 G_PER_L CHEBI:60720
sodium silicate
14 NaF
F.Na 41.988 g/mol
0.0024 G_PER_L CHEBI:28741
sodium fluoride
15 (NH4)NO3
H4N.NO3 80.043 g/mol
0.0016 G_PER_L CHEBI:63038
ammonium nitrate
16 Na2HPO4
HO4P.2Na 141.958 g/mol
0.008 G_PER_L CHEBI:34683
disodium hydrogenphosphate
17 Agar 18 G_PER_L CHEBI:2509
agar

Preparation

  1. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 514. Subsequent subculturing may be carried out in liquid medium or on agar plates.
  2. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.4 g to 1 litre water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.484620Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 514c
2026-03-13T06:19:06.152577Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001647
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.320792+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "REACTIVATION WITH LIQUID MEDIUM 514" to "reactivation_with_liquid_medium_514" for consistent matching
2026-06-05T07:41:44.098987+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.875480+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.