guyparkeria_medium

CultureMech:001650 bacterial DEFINED SOLID_AGAR
Original name: GUYPARKERIA MEDIUM

Overview

External ontology
mediadive.medium:518 (source link unavailable) — GUYPARKERIA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.3, 'max': 7.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium518.pdf

Composition

flowchart LR
    CultureMech_001650["guyparkeria_medium"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001650 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001650 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NaCl["NaCl"]
    CultureMech_001650 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001650 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_NH4Cl["NH4Cl"]
    CultureMech_001650 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Phenol_red["Phenol red"]
    CultureMech_001650 --> ing5_Phenol_red
    CHEBI_31991["CHEBI:31991"]
    ing5_Phenol_red -.-> CHEBI_31991
    ing6_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001650 --> ing6_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing6_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing7_Agar["Agar"]
    CultureMech_001650 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Na2_EDTA["Na2-EDTA"]
    CultureMech_001650 --> ing8_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing8_Na2_EDTA -.-> CHEBI_64734
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001650 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001650 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001650 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001650 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001650 --> ing13__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing13__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001650 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001650 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_NaOH["NaOH"]
    CultureMech_001650 --> ing16_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing16_NaOH -.-> CHEBI_32145

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
7.9 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
50 G_PER_L CHEBI:26710
sodium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.4 G_PER_L CHEBI:31206
ammonium chloride
6 Phenol red
C19H14O5S 354.383 g/mol
12.5 G_PER_L CHEBI:31991
phenol red
7 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
5 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
8 Agar 15 G_PER_L CHEBI:2509
agar
 Optional ingredient (for solid medium)
9 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
50 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
11 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
7.34 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
2.5 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.5 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.5 G_PER_L CHEBI:91249
ammonium molybdate
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 NaOH
HO.Na 39.997 g/mol
11 G_PER_L CHEBI:32145
sodium hydroxide

Preparation

  1. Dissolve ingredients except thiosulfate and adjust pH of medium to 7.3 - 7.5. Add thiosulfate to the medium after autoclaving from a sterile stock solution sterilized by filtration. For liquid medium also sterilize the phosphates separately in 1/10 of medium volume.
  2. Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.505036Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 518
2026-03-13T06:19:06.189492Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001650
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.361508+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GUYPARKERIA MEDIUM" to "guyparkeria_medium" for consistent matching
2026-05-20T06:01:21.414830+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5}
2026-06-05T07:41:06.808027+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.214780+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.