archaeoglobus_profundus_medium (CultureMech:001651)

Overview

External ontology

mediadive.medium:519 (source link unavailable) — ARCHAEOGLOBUS PROFUNDUS MEDIUM

Applications

Microbial cultivation

pH

6.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium519.pdf

Composition

flowchart LR
    CultureMech_001651["archaeoglobus_profundus_medium"]
    ing0_KCl["KCl"]
    CultureMech_001651 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001651 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001651 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Na2SO4["Na2SO4"]
    CultureMech_001651 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_NH4Cl["NH4Cl"]
    CultureMech_001651 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001651 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001651 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_NaCl["NaCl"]
    CultureMech_001651 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_001651 --> ing8_Yeast_extract
    ing9_Na_acetate["Na-acetate"]
    CultureMech_001651 --> ing9_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_Na_acetate -.-> CHEBI_32954
    ing10_Fe_NH4_2_SO4_2_x_7_H2O["Fe(NH4)2(SO4)2 x 7 H2O"]
    CultureMech_001651 --> ing10_Fe_NH4_2_SO4_2_x_7_H2O
    CHEBI_131378["CHEBI:131378"]
    ing10_Fe_NH4_2_SO4_2_x_7_H2O -.-> CHEBI_131378
    ing11_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001651 --> ing11_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Sodium_resazurin -.-> CHEBI_8806
    ing12_NaHCO3["NaHCO3"]
    CultureMech_001651 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001651 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001651 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001651 --> ing15_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing15_MnSO4_x_H2O -.-> CHEBI_86364
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001651 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001651 --> ing17_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001651 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001651 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001651 --> ing20_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing20_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing21_H3BO3["H3BO3"]
    CultureMech_001651 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001651 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001651 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001651 --> ing24_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001651 --> ing25_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (26)

#	Ingredient	Concentration	Ontology	Notes
1	KCl Cl.K 74.551 g/mol	0.335968 G_PER_L	CHEBI:32588 potassium chloride
2	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	3.95257 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	6.40909 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	[Merged 2 duplicates: 3.40909, 3.0]
4	Na2SO4 2Na.O4S 142.043 g/mol	2.66798 G_PER_L	CHEBI:32149 sodium sulfate
5	NH4Cl Cl.H4N 53.492 g/mol	0.247036 G_PER_L	CHEBI:31206 ammonium chloride
6	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.23834 G_PER_L	CHEBI:86158 calcium chloride dihydrate	[Merged 2 duplicates: 0.13834, 0.1]
7	K2HPO4 HO4P.2K 174.174 g/mol	0.13834 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
8	NaCl Cl.Na 58.443 g/mol	18.7866 G_PER_L	CHEBI:26710 sodium chloride	[Merged 2 duplicates: 17.7866, 1.0]
9	Yeast extract	0.494071 G_PER_L
10	Na-acetate C2H3O2.Na 82.034 g/mol	0.988142 G_PER_L	CHEBI:32954 sodium acetate
11	Fe(NH4)2(SO4)2 x 7 H2O Fe.7H2O.2H4N.2O4S 410.154 g/mol	0.00197628 G_PER_L	CHEBI:131378 ferrous ammonium sulfate heptahydrate
12	Sodium resazurin C12H7NO4 229.191 g/mol	0.000494071 G_PER_L	CHEBI:8806 Resazurin
13	NaHCO3 CHO3.Na 84.006 g/mol	0.988142 G_PER_L	CHEBI:32139 sodium hydrogencarbonate
14	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.494071 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate
15	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	1.5 G_PER_L	CHEBI:44557 nitrilotriacetic acid
16	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.5 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
17	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.1 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
18	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.18 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate
19	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.18 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate
20	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.01 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate
21	AlK(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.02 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate
22	H3BO3 H3BO3 61.833 g/mol	0.01 G_PER_L	CHEBI:33118 boric acid
23	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.01 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
24	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.03 G_PER_L	CHEBI:34887 nickel dichloride
25	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.0003 G_PER_L	CHEBI:131361 disodium selenite pentahydrate
26	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.0004 G_PER_L	CHEBI:63939 sodium tungstate dihydrate

Preparation

Dissolve ingredients except bicarbonate and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate, adjust pH to 6.5 and dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic serum vials (e.g., 20 ml medium per 100 ml bottle), pressurize bottles up to 2 bar overpressure, then autoclave. Before use, release excess pressure and reduce the medium with sulfide from a sterile anoxic stock solution prepared under 100% N2 gas.
After inoculation, add 2 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:11.507703Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 519
2026-03-13T03:09:33.692785Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 3 duplicate ingredient(s)
2026-03-13T06:19:06.200615Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001651
2026-03-17T06:30:44.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.373731+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ARCHAEOGLOBUS PROFUNDUS MEDIUM" to "archaeoglobus_profundus_medium" for consistent matching
2026-06-05T07:40:49.552660+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.472708+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.