Overview
- External ontology
- mediadive.medium:521 (source link unavailable) — DESULFOMONILE MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium521.pdf
Composition
flowchart LR
CultureMech_001654["desulfomonile_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001654 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_Na2SO4["Na2SO4"]
CultureMech_001654 --> ing1_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing1_Na2SO4 -.-> CHEBI_32149
ing2_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001654 --> ing2_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing2_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001654 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001654 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_001654 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Yeast_extract["Yeast extract"]
CultureMech_001654 --> ing6_Yeast_extract
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001654 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_NaHCO3["NaHCO3"]
CultureMech_001654 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Na_pyruvate["Na-pyruvate"]
CultureMech_001654 --> ing9_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing9_Na_pyruvate -.-> CHEBI_50144
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001654 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001654 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Riboflavin["Riboflavin"]
CultureMech_001654 --> ing12_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavin -.-> CHEBI_17015
ing13_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001654 --> ing13_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing13_Calcium_pantothenate -.-> CHEBI_31345
ing14_p_Aminobenzoate["p-Aminobenzoate"]
CultureMech_001654 --> ing14_p_Aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing14_p_Aminobenzoate -.-> CHEBI_17836
ing15_Thioctic_acid["Thioctic acid"]
CultureMech_001654 --> ing15_Thioctic_acid
CHEBI_30314["CHEBI:30314"]
ing15_Thioctic_acid -.-> CHEBI_30314
ing16_Nicotinamide["Nicotinamide"]
CultureMech_001654 --> ing16_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing16_Nicotinamide -.-> CHEBI_17154
ing17_Biotin["Biotin"]
CultureMech_001654 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Folic_acid["Folic acid"]
CultureMech_001654 --> ing18_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing18_Folic_acid -.-> CHEBI_27470
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_001654 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_Haemin["Haemin"]
CultureMech_001654 --> ing20_Haemin
CHEBI_50385["CHEBI:50385"]
ing20_Haemin -.-> CHEBI_50385
ing21_1_4_Naphthoquinone["1,4-Naphthoquinone"]
CultureMech_001654 --> ing21_1_4_Naphthoquinone
CHEBI_27418["CHEBI:27418"]
ing21_1_4_Naphthoquinone -.-> CHEBI_27418
ing22_HCl["HCl"]
CultureMech_001654 --> ing22_HCl
CHEBI_17883["CHEBI:17883"]
ing22_HCl -.-> CHEBI_17883
ing23_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001654 --> ing23_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing23_FeCl2_x_4_H2O -.-> CHEBI_86249
ing24_ZnCl2["ZnCl2"]
CultureMech_001654 --> ing24_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing24_ZnCl2 -.-> CHEBI_49976
ing25_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001654 --> ing25_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing25_MnCl2_x_4_H2O -.-> CHEBI_86368
ing26_H3BO3["H3BO3"]
CultureMech_001654 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001654 --> ing27_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing27_CoCl2_x_6_H2O -.-> CHEBI_53503
ing28_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001654 --> ing28_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing28_CuCl2_x_2_H2O -.-> CHEBI_86318
ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001654 --> ing29_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
CultureMech_001654 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 3 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 | Yeast extract | 1 G_PER_L | ||
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
2.5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.1 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.005 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 14 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 15 |
p-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.005 G_PER_L |
CHEBI:17836
4-aminobenzoate
|
|
| 16 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
|
| 17 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.05 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.005 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.005 G_PER_L |
CHEBI:27470
folic acid
|
|
| 20 | Vitamin B12 | 0.005 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 21 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.005 G_PER_L |
CHEBI:50385
hemin
|
|
| 22 |
1,4-Naphthoquinone
C10H6O2 158.156 g/mol
|
0.02 G_PER_L |
CHEBI:27418
1,4-naphthoquinone
|
|
| 23 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 24 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 25 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 26 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 29 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 30 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 32 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 33 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 34 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients (except bicarbonate, vitamins, pyruvate and sulfide), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete the medium by adding vitamins, pyruvate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and pyruvate are sterilized by filtration. Adjust pH of the complete medium to 6.8 - 7.0.
- Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.515012Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 521 |
| 2026-03-13T06:19:06.228878Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001654 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.403697+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOMONILE MEDIUM" to "desulfomonile_medium" for consistent matching |
| 2026-05-20T06:01:14.018768+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0} |
| 2026-06-05T07:40:56.817463+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.657605+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |