Overview
- External ontology
- mediadive.medium:524 (source link unavailable) — FLEXISTIPES MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium524.pdf
Composition
flowchart LR
CultureMech_001656["flexistipes_medium"]
ing0_NaCl["NaCl"]
CultureMech_001656 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001656 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_Na2SO4["Na2SO4"]
CultureMech_001656 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001656 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_001656 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_KBr["KBr"]
CultureMech_001656 --> ing5_KBr
CHEBI_32030["CHEBI:32030"]
ing5_KBr -.-> CHEBI_32030
ing6_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001656 --> ing6_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing6_SrCl2_x_6_H2O -.-> CHEBI_36385
ing7_H3BO3["H3BO3"]
CultureMech_001656 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001656 --> ing8_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing8_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing9_Na_meta_silicate["Na-meta silicate"]
CultureMech_001656 --> ing9_Na_meta_silicate
ing10_NaF["NaF"]
CultureMech_001656 --> ing10_NaF
CHEBI_28741["CHEBI:28741"]
ing10_NaF -.-> CHEBI_28741
ing11_Sodium_resazurin["Sodium resazurin"]
CultureMech_001656 --> ing11_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Sodium_resazurin -.-> CHEBI_8806
ing12_NaHCO3["NaHCO3"]
CultureMech_001656 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_Yeast_extract["Yeast extract"]
CultureMech_001656 --> ing13_Yeast_extract
ing14_KNO3["KNO3"]
CultureMech_001656 --> ing14_KNO3
CHEBI_63043["CHEBI:63043"]
ing14_KNO3 -.-> CHEBI_63043
ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001656 --> ing15_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing15_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
58.35 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
37.8 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
9.72 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
7.14 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
1.65 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
KBr
Br.K 119.002 g/mol
|
0.24 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 7 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.17 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
0.66 G_PER_L |
CHEBI:33118
boric acid
|
|
| 9 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.03 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 10 | Na-meta silicate | 0.012 G_PER_L | ||
| 11 |
NaF
F.Na 41.988 g/mol
|
0.007 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 12 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 14 | Yeast extract | 1 G_PER_L | ||
| 15 |
KNO3
K.NO3 101.102 g/mol
|
0.25 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 16 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Dissolve ingredients except bicarbonate, yeast extract, nitrate and sulfide, adjust pH to 6.0 - 6.2 using sulfuric acid and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete medium by adding yeast extract, nitrate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Adjust pH of complete medium to 6.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.522105Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 524 |
| 2026-03-13T06:19:06.246741Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001656 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.423663+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FLEXISTIPES MEDIUM" to "flexistipes_medium" for consistent matching |
| 2026-06-05T07:41:00.445020+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.815456+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:56.888316+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |