Overview
- External ontology
- mediadive.medium:529 (source link unavailable) — AMPHIBACILLUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.7
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium529.pdf
Composition
flowchart LR
CultureMech_001660["amphibacillus_medium"]
ing0_Glucose["Glucose"]
CultureMech_001660 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_K2HPO4["K2HPO4"]
CultureMech_001660 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4NO3["NH4NO3"]
CultureMech_001660 --> ing2_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing2_NH4NO3 -.-> CHEBI_63038
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001660 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_MnSO4_x_7_H2O["MnSO4 x 7 H2O"]
CultureMech_001660 --> ing4_MnSO4_x_7_H2O
CHEBI_86360["CHEBI:86360"]
ing4_MnSO4_x_7_H2O -.-> CHEBI_86360
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001660 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001660 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Yeast_extract["Yeast extract"]
CultureMech_001660 --> ing7_Yeast_extract
ing8_Polypeptone["Polypeptone"]
CultureMech_001660 --> ing8_Polypeptone
ing9_Agar["Agar"]
CultureMech_001660 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_NaHCO3["NaHCO3"]
CultureMech_001660 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Na2CO3_anhydrous["Na2CO3 anhydrous"]
CultureMech_001660 --> ing11_Na2CO3_anhydrous
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3_anhydrous -.-> CHEBI_29377
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4NO3
H4N.NO3 80.043 g/mol
|
2 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
MnSO4 x 7 H2O
Mn.O4S 151.001 g/mol
|
0.005 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.005 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 | Yeast extract | 3 G_PER_L | ||
| 9 | Polypeptone | 0.3 G_PER_L | ||
| 10 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
42 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
Na2CO3 anhydrous
CO3.2Na 105.988 g/mol
|
53 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 (see medium 31).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.543314Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 529 |
| 2026-01-28T19:48:39.024476Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:06.280069Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001660 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.460665+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AMPHIBACILLUS MEDIUM" to "amphibacillus_medium" for consistent matching |
| 2026-06-05T07:40:16.161870+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.639849+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |