modified_sme_medium

CultureMech:001664 bacterial DEFINED LIQUID
Original name: MODIFIED SME MEDIUM

Overview

External ontology
mediadive.medium:534 (source link unavailable) — MODIFIED SME MEDIUM
Applications
  • Microbial cultivation
pH
6.6
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium534.pdf

Composition

flowchart LR
    CultureMech_001664["modified_sme_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001664 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001664 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001664 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KCl["KCl"]
    CultureMech_001664 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaBr["NaBr"]
    CultureMech_001664 --> ing4_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing4_NaBr -.-> CHEBI_63004
    ing5_NH4Cl["NH4Cl"]
    CultureMech_001664 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001664 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001664 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8__NH4_2Ni_SO4_2["(NH4)2Ni(SO4)2"]
    CultureMech_001664 --> ing8__NH4_2Ni_SO4_2
    CHEBI_86149["CHEBI:86149"]
    ing8__NH4_2Ni_SO4_2 -.-> CHEBI_86149
    ing9_Na2WO4["Na2WO4"]
    CultureMech_001664 --> ing9_Na2WO4
    CHEBI_63940["CHEBI:63940"]
    ing9_Na2WO4 -.-> CHEBI_63940
    ing10_Na2SeO4["Na2SeO4"]
    CultureMech_001664 --> ing10_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing10_Na2SeO4 -.-> CHEBI_77775
    ing11_NaHCO3["NaHCO3"]
    CultureMech_001664 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001664 --> ing12_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing12_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001664 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001664 --> ing14_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing14_MnSO4_x_H2O -.-> CHEBI_86364
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001664 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001664 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001664 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001664 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001664 --> ing19_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing20_H3BO3["H3BO3"]
    CultureMech_001664 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001664 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001664 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001664 --> ing23_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
31.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 30.0, 1.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
10.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 7.0, 3.0]
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 KCl
Cl.K 74.551 g/mol
0.65 G_PER_L CHEBI:32588
potassium chloride
5 NaBr
Br.Na 102.894 g/mol
0.1 G_PER_L CHEBI:63004
sodium bromide
6 NH4Cl
Cl.H4N 53.492 g/mol
0.15 G_PER_L CHEBI:31206
ammonium chloride
7 K2HPO4
HO4P.2K 174.174 g/mol
0.15 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.5, 0.1]
9 (NH4)2Ni(SO4)2
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
10 Na2WO4
2Na.O4W 293.816 g/mol
0.01 G_PER_L CHEBI:63940
sodium tungstate
11 Na2SeO4
2Na.O4Se 188.936 g/mol
0.01 G_PER_L CHEBI:77775
sodium selenate
12 NaHCO3
CHO3.Na 84.006 g/mol
66.6667 G_PER_L CHEBI:32139
sodium hydrogencarbonate
13 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
66.6667 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
24 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Sparge medium with 100% N2 gas mixture for 20 min to make it anoxic.
  2. Adjust pH to 6.5-6.8 with 1N H2SO4.
  3. Fill 10 ml of medium in serum bottles (100 ml) under a protective H2/CO2 atmosphere and tightly stopper.
  4. Autoclave at 121 °C for 15 min.
  5. after autoclaving add the following filter-sterilized solutions.
  6. apply xx bar H2/CO2 (80%/20%) + yy % O2 (Note: strain specific conditions need to be applied)
  7. For more details on the medium see the original description (Huber et al. System. Appl. Microbiol. 15:340-351 (1992)).
  8. To 1000.0 ml trace element solution in medium 141 add:
  9. prepared under CO2 in an ice-bath, filter-sterilise
  10. Prepared under N2,autoclave 121°C, 15 min
  11. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.549892Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 534
2026-03-13T03:09:42.591574Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:06.311806Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001664
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.495134+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED SME MEDIUM" to "modified_sme_medium" for consistent matching
2026-06-05T07:41:37.743842+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.654498+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:08.937898+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.