reactivation_with_liquid_medium (CultureMech:001667)

Overview

External ontology

mediadive.medium:535b (source link unavailable) — REACTIVATION WITH LIQUID MEDIUM

Applications

Microbial cultivation

pH

7.3

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535b.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001667["reactivation_with_liquid_medium"]
    ing0_Casein_peptone["Casein peptone"]
    CultureMech_001667 --> ing0_Casein_peptone
    ing1_Soy_peptone["Soy peptone"]
    CultureMech_001667 --> ing1_Soy_peptone
    ing2_D____Glucose["D(+)-Glucose"]
    CultureMech_001667 --> ing2_D____Glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_D____Glucose -.-> CHEBI_17634
    ing3_NaCl["NaCl"]
    CultureMech_001667 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_K2HPO4["K2HPO4"]
    CultureMech_001667 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Agar["Agar"]
    CultureMech_001667 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001667 --> ing6_Pancreatic_digest_of_casein
    ing7_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001667 --> ing7_Peptic_digest_of_soybean_meal
    ing8_Glucose["Glucose"]
    CultureMech_001667 --> ing8_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_Glucose -.-> CHEBI_17234
    ing9_Sodium_chloride["Sodium chloride"]
    CultureMech_001667 --> ing9_Sodium_chloride
    ing9_Sodium_chloride -.-> CHEBI_26710
    ing10_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001667 --> ing10_Dipotassium_phosphate
    ing10_Dipotassium_phosphate -.-> CHEBI_131527
    ing11_Agar["Agar"]
    CultureMech_001667 --> ing11_Agar
    ing11_Agar -.-> CHEBI_2509

Ingredients (12)

#	Ingredient	Concentration	Ontology	Notes
1	Casein peptone	17 G_PER_L
2	Soy peptone	3 G_PER_L
3	D(+)-Glucose C6H12O6 180.156 g/mol	2.5 G_PER_L	CHEBI:17634 D-glucose
4	NaCl Cl.Na 58.443 g/mol	5 G_PER_L	CHEBI:26710 sodium chloride
5	K2HPO4 HO4P.2K 174.174 g/mol	2.5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
6	Agar	35.0 G_PER_L	CHEBI:2509 agar	[Merged 2 duplicates: 15.0, 20.0]
7	Pancreatic digest of casein Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	17.0 G_PER_L	MICRO:0000182 (source link unavailable) tryptone	Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
8	Peptic digest of soybean meal Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	3.0 G_PER_L	FOODON:03315720 vegetable protein, hydrolyzed	Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
9	Glucose C6H12O6 180.156 g/mol Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	2.5 G_PER_L	CHEBI:17234 glucose	Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
10	Sodium chloride Cl.Na 58.443 g/mol Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	5.0 G_PER_L	CHEBI:26710 sodium chloride	Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
11	Dipotassium phosphate HO4P.2K 174.174 g/mol Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	2.5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
12	Agar Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	15.0 G_PER_L	CHEBI:2509 agar	Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product

Preparation

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium 545 or on agar plates.
Adjust pH to 7.3.
Medium 545 is the same as Caso Bouillon (Merck or Oxoid, Germany).
pH 7.3
Autoclave at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:11.556363Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 535b
2026-03-13T03:08:38.105371Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:19:06.324846Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001667
2026-03-17T06:29:39.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.509846+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "REACTIVATION WITH LIQUID MEDIUM" to "reactivation_with_liquid_medium" for consistent matching
2026-06-05T07:41:44.061379+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.870739+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.