herbaspirillum_medium

CultureMech:001670 bacterial COMPLEX SOLID_AGAR
Original name: HERBASPIRILLUM MEDIUM

Overview

External ontology
mediadive.medium:538 (source link unavailable) — HERBASPIRILLUM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium538.pdf

Composition

flowchart LR
    CultureMech_001670["herbaspirillum_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001670 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001670 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001670 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaCl["NaCl"]
    CultureMech_001670 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2["CaCl2"]
    CultureMech_001670 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_FeCl2_x_6_H2O["FeCl2 x 6 H2O"]
    CultureMech_001670 --> ing5_FeCl2_x_6_H2O
    CHEBI_30812["CHEBI:30812"]
    ing5_FeCl2_x_6_H2O -.-> CHEBI_30812
    ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001670 --> ing6_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_001670 --> ing7_Yeast_extract
    ing8_Agar["Agar"]
    CultureMech_001670 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Na_malate["Na-malate"]
    CultureMech_001670 --> ing9_Na_malate
    CHEBI_91260["CHEBI:91260"]
    ing9_Na_malate -.-> CHEBI_91260

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NaCl
Cl.Na 58.443 g/mol
0.1 G_PER_L CHEBI:26710
sodium chloride
5 CaCl2
Ca.2Cl 110.984 g/mol
0.02 G_PER_L CHEBI:3312
calcium dichloride
6 FeCl2 x 6 H2O
Cl2Fe 126.751 g/mol
0.01 G_PER_L CHEBI:30812
iron dichloride
7 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.002 G_PER_L CHEBI:75213
sodium molybdate dihydrate
8 Yeast extract 0.05 G_PER_L
9 Agar 15 G_PER_L CHEBI:2509
agar
10 Na-malate 100 G_PER_L CHEBI:91260
disodium malate

Preparation

  1. pH 7.0
  2. Autoclave at 121°C for 15 min and after sterilization add filter sterilized solution A.
  3. pH 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.564124Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 538
2026-03-13T06:19:06.340332Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001670
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.526438+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HERBASPIRILLUM MEDIUM" to "herbaspirillum_medium" for consistent matching
2026-06-05T07:41:08.430888+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.740042+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.