Overview
- External ontology
- mediadive.medium:544 (source link unavailable) — NZCYM-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium544.pdf
Composition
flowchart LR
CultureMech_001676["nzcym_medium"]
ing0_Casein_hydrolysate["Casein hydrolysate"]
CultureMech_001676 --> ing0_Casein_hydrolysate
ing1_NaCl["NaCl"]
CultureMech_001676 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Casamino_acids["Casamino acids"]
CultureMech_001676 --> ing2_Casamino_acids
ing3_Yeast_extract["Yeast extract"]
CultureMech_001676 --> ing3_Yeast_extract
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001676 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Maltose["Maltose"]
CultureMech_001676 --> ing5_Maltose
CHEBI_17306["CHEBI:17306"]
ing5_Maltose -.-> CHEBI_17306
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein hydrolysate | 10 G_PER_L | ||
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 | Casamino acids | 1 G_PER_L | ||
| 4 | Yeast extract | 5 G_PER_L | ||
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
Maltose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:17306
maltose
|
Preparation
- Adjust pH to 7.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.579013Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 544 |
| 2026-01-28T19:48:38.157199Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:06.388354Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001676 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.578949+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NZCYM-MEDIUM" to "nzcym_medium" for consistent matching |
| 2026-06-05T07:41:40.274764+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.461814+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |