diluted_tryptone_soya_broth_tsb

CultureMech:001678 bacterial COMPLEX LIQUID
Original name: DILUTED TRYPTONE SOYA BROTH (TSB)

Overview

External ontology
mediadive.medium:545a (source link unavailable) — DILUTED TRYPTONE SOYA BROTH (TSB)
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium545a.pdf

Composition

flowchart LR
    CultureMech_001678["diluted_tryptone_soya_broth_tsb"]
    ing0_Casein_peptone["Casein peptone"]
    CultureMech_001678 --> ing0_Casein_peptone
    ing1_Soy_peptone["Soy peptone"]
    CultureMech_001678 --> ing1_Soy_peptone
    ing2_D____Glucose["D(+)-Glucose"]
    CultureMech_001678 --> ing2_D____Glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_D____Glucose -.-> CHEBI_17634
    ing3_NaCl["NaCl"]
    CultureMech_001678 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_K2HPO4["K2HPO4"]
    CultureMech_001678 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Casein peptone 17 G_PER_L
2 Soy peptone 3 G_PER_L
3 D(+)-Glucose
C6H12O6 180.156 g/mol
2.5 G_PER_L CHEBI:17634
D-glucose
4 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
5 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate

Preparation

  1. Dilute medium 545 1:10.
  2. Adjust pH to 7.3.
  3. Note: The following brands are used at the DSMZ: Difco™ Trypton-Soja-Bouillon (Soja-Caseinpepton-Bouillon) BD 211825. BBL™ Trypticase Soja-Bouillon (Caseinpepton-Soja-Bouillon) BD 211768. Oxoid Tryptone Soy Broth (Casein soya bean digest medium) CM0129 Merck Tryptic Soy Broth (CASO-Bouillon) 105459

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.581644Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 545a
2026-03-13T06:19:06.395809Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001678
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.587105+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DILUTED TRYPTONE SOYA BROTH (TSB)" to "diluted_tryptone_soya_broth_tsb" for consistent matching
2026-06-05T07:40:58.741049+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.385183+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.