Overview
- External ontology
- mediadive.medium:547 (source link unavailable) — ISP MEDIUM 4
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium547.pdf
Composition
flowchart LR
CultureMech_001681["isp_medium_4"]
ing0_Agar["Agar"]
CultureMech_001681 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1_Starch["Starch"]
CultureMech_001681 --> ing1_Starch
CHEBI_28017["CHEBI:28017"]
ing1_Starch -.-> CHEBI_28017
ing2_CaCO3["CaCO3"]
CultureMech_001681 --> ing2_CaCO3
CHEBI_3311["CHEBI:3311"]
ing2_CaCO3 -.-> CHEBI_3311
ing3_K2HPO4["K2HPO4"]
CultureMech_001681 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001681 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_NaCl["NaCl"]
CultureMech_001681 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_001681 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 19.98 G_PER_L |
CHEBI:2509
agar
|
|
| 2 | Starch | 20 G_PER_L |
CHEBI:28017
starch
|
|
| 3 |
CaCO3
CO3.Ca 100.086 g/mol
|
3.99202 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.99601 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.99601 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
1.99601 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3.99202 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Preparation
- The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.
- Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.
Solutions
Trace salt solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeSO4 x 7 H2O | 1 G_PER_L | |
| MnCl2 x 4 H2O | 1 G_PER_L | |
| ZnSO4 x 7 H2O | 1 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.585934Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 547 |
| 2026-03-13T06:19:06.409213Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001681 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.601981+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ISP MEDIUM 4" to "isp_medium_4" for consistent matching |
| 2026-06-05T07:41:09.707328+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.154361+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:22.386780+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace salt solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |