clostridium_sp_medium

CultureMech:001685 bacterial COMPLEX SOLID_AGAR
Original name: CLOSTRIDIUM SP. MEDIUM

Overview

External ontology
mediadive.medium:54b (source link unavailable) — CLOSTRIDIUM SP. MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium54b.pdf

Composition

flowchart LR
    CultureMech_001685["clostridium_sp_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_001685 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001685 --> ing1_Yeast_extract
    ing2_CaCO3["CaCO3"]
    CultureMech_001685 --> ing2_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing2_CaCO3 -.-> CHEBI_3311
    ing3_Agar["Agar"]
    CultureMech_001685 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
2 Yeast extract 10 G_PER_L
3 CaCO3
CO3.Ca 100.086 g/mol
20 G_PER_L CHEBI:3311
calcium carbonate
4 Agar 17 G_PER_L CHEBI:2509
agar
 Optional ingredient (for solid medium)

Preparation

  1. Dissolve ingredients (note that most of the carbonate remains suspended) and sparge medium with 100% N2 gas for 30 -45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials vials while stirring to keep carbonate suspended and autoclave.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.057000Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 54b
2026-03-13T06:19:06.427902Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001685
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.621448+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CLOSTRIDIUM SP. MEDIUM" to "clostridium_sp_medium" for consistent matching
2026-06-05T07:40:54.207265+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.