cyc_medium_modified_following_cross_and_attwell_1973

CultureMech:001686 bacterial COMPLEX SOLID_AGAR
Original name: CYC-MEDIUM (modified following Cross and Attwell,1973)

Overview

External ontology
mediadive.medium:550 (source link unavailable) — CYC-MEDIUM (modified following Cross and Attwell,1973)
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium550.pdf

Composition

flowchart LR
    CultureMech_001686["cyc_medium_modified_following_cross_and_attwell_1973"]
    ing0_Czapek_Dox_agar["Czapek Dox agar"]
    CultureMech_001686 --> ing0_Czapek_Dox_agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Czapek_Dox_agar -.-> CHEBI_2509
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001686 --> ing1_Yeast_extract
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_001686 --> ing2_Casamino_acids
    ing3_Tryptophan["Tryptophan"]
    CultureMech_001686 --> ing3_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing3_Tryptophan -.-> CHEBI_27897
    ing4_Agar["Agar"]
    CultureMech_001686 --> ing4_Agar
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Czapek Dox agar 48 G_PER_L CHEBI:2509
agar
2 Yeast extract 2 G_PER_L
3 Casamino acids 6.1 G_PER_L
4 Tryptophan
C11H12N2O2 204.229 g/mol
0.02 G_PER_L CHEBI:27897
tryptophan
5 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. pH 7.2

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.590665Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 550
2026-01-28T19:48:12.585288Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:06.431520Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001686
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.625433+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CYC-MEDIUM (modified following Cross and Attwell,1973)" to "cyc_medium_modified_following_cross_and_attwell_1973" for consistent matching
2026-06-05T07:40:55.152314+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:36.496416+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.