sulfuriferula_medium

CultureMech:001696 bacterial DEFINED LIQUID
Original name: SULFURIFERULA MEDIUM

Overview

External ontology
mediadive.medium:561 (source link unavailable) — SULFURIFERULA MEDIUM
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium561.pdf

Composition

flowchart LR
    CultureMech_001696["sulfuriferula_medium"]
    ing0_KCl["KCl"]
    CultureMech_001696 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001696 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001696 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001696 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001696 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_K2HPO4["K2HPO4"]
    CultureMech_001696 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_NaCl["NaCl"]
    CultureMech_001696 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001696 --> ing7_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing7_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing8_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001696 --> ing8_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing8_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001696 --> ing9_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing10_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001696 --> ing10_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing10_MnSO4_x_H2O -.-> CHEBI_86364
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001696 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001696 --> ing12_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing12_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001696 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001696 --> ing14_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing15_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001696 --> ing15_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing15_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing16_H3BO3["H3BO3"]
    CultureMech_001696 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001696 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001696 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 KCl
Cl.K 74.551 g/mol
0.295566 G_PER_L CHEBI:32588
potassium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.70936 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.3990100000000005 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.39901, 3.0]
4 NH4Cl
Cl.H4N 53.492 g/mol
1.23153 G_PER_L CHEBI:31206
ammonium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.237931 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.137931, 0.1]
6 K2HPO4
HO4P.2K 174.174 g/mol
0.137931 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 NaCl
Cl.Na 58.443 g/mol
1.4926110000000001 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.492611, 1.0]
8 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.034926109999999996 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.00492611, 0.03]
9 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.000892611 G_PER_L CHEBI:63939
sodium tungstate dihydrate
 [Merged 2 duplicates: 0.000492611, 0.0004]
10 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
11 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
14 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
15 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
16 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Dissolve ingredients, adjust pH to 6.0 with H2SO4 and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into serum vials (e.g., 20 ml medium in 100 ml bottles) and autoclave. Adjust medium pH to 6.0, if necessary.
  2. After inoculation, add by injection 3% (v/v) sterile air (usually 9 ml per bottle) and pressurize bottles with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.617663Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 561
2026-03-13T03:08:51.501149Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:06.523332Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001696
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.727772+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFURIFERULA MEDIUM" to "sulfuriferula_medium" for consistent matching
2026-06-05T07:41:47.648791+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:12.100045+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.