medium_125_with_methanol

CultureMech:001698 bacterial DEFINED SOLID_AGAR
Original name: MEDIUM 125 WITH METHANOL

Overview

External ontology
mediadive.medium:569 (source link unavailable) — MEDIUM 125 WITH METHANOL
Applications
  • Microbial cultivation
pH range
{'min': 4.0, 'max': 5.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium569.pdf

Composition

flowchart LR
    CultureMech_001698["medium_125_with_methanol"]
    ing0_Methanol["Methanol"]
    CultureMech_001698 --> ing0_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing0_Methanol -.-> CHEBI_17790
    ing1_KNO3["KNO3"]
    CultureMech_001698 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001698 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001698 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Na2HPO4["Na2HPO4"]
    CultureMech_001698 --> ing4_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing4_Na2HPO4 -.-> CHEBI_34683
    ing5_NaH2PO4["NaH2PO4"]
    CultureMech_001698 --> ing5_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing5_NaH2PO4 -.-> CHEBI_37585
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001698 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001698 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_H3BO3["H3BO3"]
    CultureMech_001698 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
    CultureMech_001698 --> ing9_MnSO4_x_5_H2O
    CHEBI_131524["CHEBI:131524"]
    ing9_MnSO4_x_5_H2O -.-> CHEBI_131524
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001698 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MoO3["MoO3"]
    CultureMech_001698 --> ing11_MoO3
    CHEBI_30627["CHEBI:30627"]
    ing11_MoO3 -.-> CHEBI_30627
    ing12_Agar["Agar"]
    CultureMech_001698 --> ing12_Agar
    CHEBI_2509["CHEBI:2509"]
    ing12_Agar -.-> CHEBI_2509

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
2 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 Na2HPO4
HO4P.2Na 141.958 g/mol
0.23 G_PER_L CHEBI:34683
disodium hydrogenphosphate
6 NaH2PO4
H2O4P.Na 119.976 g/mol
0.07 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.001 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
5e-06 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
9 H3BO3
H3BO3 61.833 g/mol
1e-05 G_PER_L CHEBI:33118
boric acid
10 MnSO4 x 5 H2O
5H2O.Mn.O4S 241.076 g/mol
1e-05 G_PER_L CHEBI:131524
manganese(II) sulfate pentahydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
7e-05 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MoO3
MoO3 143.937 g/mol
1e-05 G_PER_L CHEBI:30627
molybdenum trioxide
13 Agar 12 G_PER_L CHEBI:2509
agar

Preparation

  1. To 1 l of medium 125 pH adjusted to 4.0-5.4 add 10 ml CH3OH.
  2. Add 5 ml methanol or incubate under methane-air (4:1). Adjust pH to 6.8.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.623348Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 569
2026-03-13T06:19:06.534827Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001698
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.740265+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM 125 WITH METHANOL" to "medium_125_with_methanol" for consistent matching
2026-05-20T06:01:45.268217+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '4-5.4' -> {'min': 4.0, 'max': 5.4}
2026-06-05T07:41:15.061381+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.108914+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:01.556068+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.