Overview
- External ontology
- mediadive.medium:57 (source link unavailable) — AAM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium57.pdf
Composition
flowchart LR
CultureMech_001706["aam_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_001706 --> ing0_Tryptone
ing1_Meat_extract["Meat extract"]
CultureMech_001706 --> ing1_Meat_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_001706 --> ing2_Yeast_extract
ing3_Tween_80["Tween 80"]
CultureMech_001706 --> ing3_Tween_80
CHEBI_53426["CHEBI:53426"]
ing3_Tween_80 -.-> CHEBI_53426
ing4_Na_acetate["Na-acetate"]
CultureMech_001706 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001706 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001706 --> ing6_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing6_MnSO4_x_H2O -.-> CHEBI_86364
ing7_Casamino_acids["Casamino acids"]
CultureMech_001706 --> ing7_Casamino_acids
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 4.99501 G_PER_L | ||
| 2 | Meat extract | 4.99501 G_PER_L | ||
| 3 | Yeast extract | 6.99301 G_PER_L | ||
| 4 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
2.4975 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1998 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.04995 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 8 | Casamino acids | 6.99301 G_PER_L |
Preparation
- Adjust pH to 5.4.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.061185Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 57 |
| 2026-01-28T19:48:17.812009Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:06.588658Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001706 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.798143+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AAM MEDIUM" to "aam_medium" for consistent matching |
| 2026-06-05T07:40:46.523232+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.399383+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:52.317856+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |