Overview
- External ontology
- mediadive.medium:582 (source link unavailable) — 15% MH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium582.pdf
Composition
flowchart LR
CultureMech_001708["15_mh_medium"]
ing0_NaCl["NaCl"]
CultureMech_001708 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2["MgCl2"]
CultureMech_001708 --> ing1_MgCl2
CHEBI_6636["CHEBI:6636"]
ing1_MgCl2 -.-> CHEBI_6636
ing2_MgSO4["MgSO4"]
CultureMech_001708 --> ing2_MgSO4
CHEBI_32599["CHEBI:32599"]
ing2_MgSO4 -.-> CHEBI_32599
ing3_CaCl2["CaCl2"]
CultureMech_001708 --> ing3_CaCl2
CHEBI_3312["CHEBI:3312"]
ing3_CaCl2 -.-> CHEBI_3312
ing4_KCl["KCl"]
CultureMech_001708 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaHCO3["NaHCO3"]
CultureMech_001708 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_NaBr["NaBr"]
CultureMech_001708 --> ing6_NaBr
CHEBI_63004["CHEBI:63004"]
ing6_NaBr -.-> CHEBI_63004
ing7_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_001708 --> ing7_Proteose_peptone_no__3
ing8_Yeast_extract["Yeast extract"]
CultureMech_001708 --> ing8_Yeast_extract
ing9_Glucose["Glucose"]
CultureMech_001708 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
121.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2
2Cl.Mg 95.211 g/mol
|
10.5 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 3 |
MgSO4
Mg.O4S 120.368 g/mol
|
14.4 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 4 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.54 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.09 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
NaBr
Br.Na 102.894 g/mol
|
0.039 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 8 | Proteose peptone no. 3 | 5 G_PER_L | ||
| 9 | Yeast extract | 10 G_PER_L | ||
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Preparation
- Final pH 7.5
- NaHCO3 is added from a sterilized stock solution when the medium has cooled. Agar is added at a concentration of 20 g/l for solid media.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.643412Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 582 |
| 2026-03-13T06:19:06.598209Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001708 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.807643+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "15% MH MEDIUM" to "15_mh_medium" for consistent matching |
| 2026-06-05T07:40:15.297908+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.446967+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |