Overview
- External ontology
- mediadive.medium:584 (source link unavailable) — BRUCELLA AGAR
- Applications
-
- Microbial cultivation
- Notes
- DSMZ Medium 584 directs preparing Brucella agar, cooling it to 50 C after autoclaving, and adding 100 ml/L sheep blood.
Composition
flowchart LR
CultureMech_001710["brucella_agar"]
ing0_Brucella_agar["Brucella agar"]
CultureMech_001710 --> ing0_Brucella_agar
ing1_Sheep_blood["Sheep blood"]
CultureMech_001710 --> ing1_Sheep_blood
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Brucella agar | 1000 ML_PER_L |
MICRO:0000595 (source link unavailable)
Brucella agar
|
DSMZ Medium 584 says to prepare Brucella agar, e.g. from Merck, before adding sheep blood. |
| 2 | Sheep blood | 100 ML_PER_L |
UBERON:0000178
blood
|
DSMZ Medium 584 adds 100 ml/L sheep blood. |
Preparation
- Prepare Brucella agar according to the manufacturer's directions.
- Autoclave the Brucella agar.
- Cool the autoclaved Brucella agar to 50 C.
- Add 100 ml/L sheep blood.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.646317Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 584 |
| 2026-03-13T06:19:06.604656Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001710 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.815007+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BRUCELLA AGAR" to "brucella_agar" for consistent matching |
| 2026-06-05T07:40:51.572096+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_official_simple_score20.py | RESOLVED_OFFICIAL_SIMPLE_SCORE20 | DSMZ Medium 584 directs preparing Brucella agar, cooling it to 50 C after autoclaving, and adding 100 ml/L sheep blood. |