bcye_alpha_agar

Overview

External ontology

mediadive.medium:585a (source link unavailable) — BCYE alpha agar

Applications

• Microbial cultivation

pH

6.9

Notes

Source: DSMZ

Composition

flowchart LR
    CultureMech_001712["bcye_alpha_agar"]
    ing0_OXOID_Legionella_CYE_Agar_base["OXOID Legionella CYE-Agar base"]
    CultureMech_001712 --> ing0_OXOID_Legionella_CYE_Agar_base
    ing1_ACES["ACES"]
    CultureMech_001712 --> ing1_ACES
    CHEBI_39061["CHEBI:39061"]
    ing1_ACES -.-> CHEBI_39061
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001712 --> ing2_Yeast_extract
    ing3_Activated_charcoal["Activated charcoal"]
    CultureMech_001712 --> ing3_Activated_charcoal
    ing4_Na2_alpha_ketoglutarate["Na2 alpha-ketoglutarate"]
    CultureMech_001712 --> ing4_Na2_alpha_ketoglutarate
    CHEBI_16810["CHEBI:16810"]
    ing4_Na2_alpha_ketoglutarate -.-> CHEBI_16810
    ing5_Agar["Agar"]
    CultureMech_001712 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001712 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Fe4_PO4_2["Fe4(PO4)2"]
    CultureMech_001712 --> ing7_Fe4_PO4_2
    CHEBI_132767["CHEBI:132767"]
    ing7_Fe4_PO4_2 -.-> CHEBI_132767

Ingredients (8)

Preparation

1. Prepare OXOID Legionella CYE-Agar base or solution A.
2. Cool down to 50 °C.
3. Add solution C (Cysteine-HCl) and solution D (ferric pyrophosphate).
4. Adjust pH to 6.9 with sterile 1.0 N KOH or HCl.
5. The pH of the medium is critical.
6. Finally, add solution B. Swirl medium in flask during dispensing to petri dishes or tubes in order to keep charcoal suspended.
7. Dehydrated media preparations are available from media distributors.
8. We use OXOID Legionella CYE-Agar base with Legionella BCYE supplement.
9. Note: Alternatively use CYE-ACES AGAR
10. Dissolve ACES and bring to pH 6.9 by slowly adding 1 N KOH. Add the other components and dissolve by boiling. Autoclave at 121°C for 15 minutes. Cool to 50 - 55°C.
11. Autoclave at 121°C and cool to 50 - 55°C.
12. Prepare a fresh solution and filter-sterilize it.
13. Prepare a fresh solution by heating to 50 - 55°C in a water-bath. Filter-sterilize solution separately.
14. Please note: Soluble ferric pyrophosphate must be kept dry and in the dark. Do not use if chemical looses its green colour and becomes brown or yellow.

Target organisms

No target organisms are recorded.

Provenance