Overview
- External ontology
- mediadive.medium:589 (source link unavailable) — HALOBACTERIUM LACUSPROFUNDII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium589.pdf
Composition
flowchart LR
CultureMech_001716["halobacterium_lacusprofundii_medium"]
ing0_NaCl["NaCl"]
CultureMech_001716 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001716 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001716 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_001716 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001716 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Yeast_extract["Yeast extract"]
CultureMech_001716 --> ing5_Yeast_extract
ing6_Sodium_succinate["Sodium succinate"]
CultureMech_001716 --> ing6_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing6_Sodium_succinate -.-> CHEBI_63675
ing7_water__distilled_or_tapwater_["water (distilled or tapwater)"]
CultureMech_001716 --> ing7_water__distilled_or_tapwater_
ing8_Agar["Agar"]
CultureMech_001716 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
181 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
7.4 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Yeast extract | 1 G_PER_L | ||
| 7 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
10 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 8 | water (distilled or tapwater) | 1000 G_PER_L | ||
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- pH 7.4
- Filter-sterilize vitamin solution and add to the medium when it has cooled to about 55 - 60°C. Add agar at a concentration of 15 g/l for solid media.
Solutions
Vitamin solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 0.1 G_PER_L | |
| Vitamin B12 | 0.1 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.1 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.659281Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 589 |
| 2026-03-13T06:19:06.645750Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001716 |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.857075+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOBACTERIUM LACUSPROFUNDII MEDIUM" to "halobacterium_lacusprofundii_medium" for consistent matching |
| 2026-06-05T07:41:07.595933+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-07T07:05:21.407062+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Vitamin solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |