Overview
- External ontology
- mediadive.medium:595 (source link unavailable) — CAULOBACTER MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium595.pdf
Composition
flowchart LR
CultureMech_001722["caulobacter_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_001722 --> ing0_Bacto_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001722 --> ing1_Yeast_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001722 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_Tap_water["Tap water"]
CultureMech_001722 --> ing3_Tap_water
CHEBI_15377["CHEBI:15377"]
ing3_Tap_water -.-> CHEBI_15377
ing4_Agar["Agar"]
CultureMech_001722 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 2 G_PER_L | ||
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
Tap water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Add 15.0 g/l agar for solid medium.
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Caulobacter sp. K31
- Term
- Id
- NCBITaxon:366602
- Label
- Caulobacter sp. K31
- Growth metrics
- Doubling time minutes
- 2400.0
- Evidence
- Reference
- PMID:25274120
- Supports
- SUPPORT
- Snippet
- K31 is a novel Caulobacter isolate that has the ability to tolerate copper and chlorophenols, and can grow at 4 ° C with a doubling time of 40 h.
- Explanation
- Auto-applied from literature query: backfill PMID:25274120
- Is max attainment
- False
- Growth mode
- BATCH
- Perturbations
- Perturbation type
- TEMPERATURE_STRESS
- Descriptor
- cold growth at 4°C
- Target
- temperature
- Level
- 4.0
- Level unit
- °C
Recipe variants
- Name
- cold_growth_caulobacter_k31
- Description
- Cold-growth condition for Caulobacter sp. K31 on Caulobacter Medium.
- Modifications
- Incubate at 4 C.
- Use Caulobacter sp. K31 as the target strain.
- Purpose
- Capture strain-specific cold-growth evidence under the parent Caulobacter Medium record.
- Evidence
- Reference
- PMID:25274120
- Supports
- SUPPORT
- Snippet
- K31 is a novel Caulobacter isolate that has the ability to tolerate copper and chlorophenols, and can grow at 4 ° C with a doubling time of 40 h.
- Explanation
- Literature evidence supports a cold-growth variant for Caulobacter sp. K31 on the parent Caulobacter Medium record.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.675863Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 595 |
| 2026-03-13T06:19:06.693555Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001722 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.908067+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CAULOBACTER MEDIUM" to "caulobacter_medium" for consistent matching |
| 2026-05-04T06:34:23.033204+00:00 | literature_review_apply | ADDED_GROWTH_EVIDENCE | organisms_added=1 metrics_added=1 genomes_added=0 strain_mods_added=0 |
| 2026-05-05T07:23:23.120055+00:00 | literature_review_apply | ADDED_GROWTH_EVIDENCE | organisms_added=1 metrics_added=1 genomes_added=0 |
| 2026-06-05T07:40:52.727347+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T00:58:02.723427+00:00 | curate_organism_culture_type.py | SET_ORGANISM_CULTURE_TYPE | Inferred isolate: 1 specific strain(s) named, no community signal (#142) |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |