Overview
- External ontology
- mediadive.medium:596 (source link unavailable) — BLASTOCOCCUS AGGREGATUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium596.pdf
Composition
flowchart LR
CultureMech_001723["blastococcus_aggregatus_medium"]
ing0_KNO3["KNO3"]
CultureMech_001723 --> ing0_KNO3
CHEBI_63043["CHEBI:63043"]
ing0_KNO3 -.-> CHEBI_63043
ing1_Sodium_glycerophosphate["Sodium glycerophosphate"]
CultureMech_001723 --> ing1_Sodium_glycerophosphate
ing2_Tris_HCl["Tris HCl"]
CultureMech_001723 --> ing2_Tris_HCl
CHEBI_9754["CHEBI:9754"]
ing2_Tris_HCl -.-> CHEBI_9754
ing3_Tryptone["Tryptone"]
CultureMech_001723 --> ing3_Tryptone
ing4_Yeast_extract["Yeast extract"]
CultureMech_001723 --> ing4_Yeast_extract
ing5_Sea_water["Sea water"]
CultureMech_001723 --> ing5_Sea_water
ing6_Na_tartrate["Na-tartrate"]
CultureMech_001723 --> ing6_Na_tartrate
CHEBI_63017["CHEBI:63017"]
ing6_Na_tartrate -.-> CHEBI_63017
ing7_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001723 --> ing7_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing7_CuCl2_x_2_H2O -.-> CHEBI_86318
ing8_ZnCl2["ZnCl2"]
CultureMech_001723 --> ing8_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing8_ZnCl2 -.-> CHEBI_49976
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001723 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001723 --> ing10_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO3
K.NO3 101.102 g/mol
|
0.5 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 2 | Sodium glycerophosphate | 0.1 G_PER_L | ||
| 3 |
Tris HCl
C4H11NO3 121.136 g/mol
|
1 G_PER_L |
CHEBI:9754
tris
|
|
| 4 | Tryptone | 2 G_PER_L | ||
| 5 | Yeast extract | 2 G_PER_L | ||
| 6 | Sea water | 999 G_PER_L | ||
| 7 |
Na-tartrate
C4H4O6.2Na 194.05 g/mol
|
1.77 G_PER_L |
CHEBI:63017
sodium L-tartrate
|
|
| 8 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0269 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 9 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0208 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0404 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0252 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- pH 7.0
- Artificial sea water is prepared from a marine aquarium salts mixture.
Solutions
Trace elements
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| H3BO3 | 2.85 G_PER_L | |
| MnCl2 x 4 H2O | 1.8 G_PER_L | |
| FeSO4 | 1.36 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.677089Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 596 |
| 2026-02-06T05:10:28.632182Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:06.699315Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001723 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.913901+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BLASTOCOCCUS AGGREGATUS MEDIUM" to "blastococcus_aggregatus_medium" for consistent matching |
| 2026-06-05T07:40:51.115111+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.589831+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:53.918697+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:21.658475+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace elements' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |