Overview
- External ontology
- mediadive.medium:600 (source link unavailable) — M14 (PIRELLULA MARINA) MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium600.pdf
Composition
flowchart LR
CultureMech_001727["m14_pirellula_marina_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001727 --> ing0_Yeast_extract
ing1_Glucose["Glucose"]
CultureMech_001727 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_001727 --> ing2_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing2_Tris_HCl_buffer -.-> CHEBI_9754
ing3_Vitamin_B12["Vitamin B12"]
CultureMech_001727 --> ing3_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing3_Vitamin_B12 -.-> CHEBI_176843
ing4_Biotin["Biotin"]
CultureMech_001727 --> ing4_Biotin
CHEBI_15956["CHEBI:15956"]
ing4_Biotin -.-> CHEBI_15956
ing5_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001727 --> ing5_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing5_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing6_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001727 --> ing6_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing6_Calcium_pantothenate -.-> CHEBI_31345
ing7_Folic_acid["Folic acid"]
CultureMech_001727 --> ing7_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing7_Folic_acid -.-> CHEBI_27470
ing8_Riboflavin["Riboflavin"]
CultureMech_001727 --> ing8_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing8_Riboflavin -.-> CHEBI_17015
ing9_Nicotinamide["Nicotinamide"]
CultureMech_001727 --> ing9_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing9_Nicotinamide -.-> CHEBI_17154
ing10_NaCl["NaCl"]
CultureMech_001727 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_Na2SO4["Na2SO4"]
CultureMech_001727 --> ing11_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing11_Na2SO4 -.-> CHEBI_32149
ing12_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001727 --> ing12_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing12_MgCl2_x_6_H2O -.-> CHEBI_86345
ing13_CaCl2["CaCl2"]
CultureMech_001727 --> ing13_CaCl2
CHEBI_3312["CHEBI:3312"]
ing13_CaCl2 -.-> CHEBI_3312
ing14_NaHCO3["NaHCO3"]
CultureMech_001727 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
ing15_KCl["KCl"]
CultureMech_001727 --> ing15_KCl
CHEBI_32588["CHEBI:32588"]
ing15_KCl -.-> CHEBI_32588
ing16_KBr["KBr"]
CultureMech_001727 --> ing16_KBr
CHEBI_32030["CHEBI:32030"]
ing16_KBr -.-> CHEBI_32030
ing17_H3BO3["H3BO3"]
CultureMech_001727 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_SrCl2["SrCl2"]
CultureMech_001727 --> ing18_SrCl2
CHEBI_36383["CHEBI:36383"]
ing18_SrCl2 -.-> CHEBI_36383
ing19_NaF["NaF"]
CultureMech_001727 --> ing19_NaF
CHEBI_28741["CHEBI:28741"]
ing19_NaF -.-> CHEBI_28741
ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001727 --> ing20_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
ing21_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001727 --> ing21_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing21_MgSO4_x_7_H2O -.-> CHEBI_31795
ing22_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001727 --> ing22_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing22_CaCl2_x_2_H2O -.-> CHEBI_86158
ing23__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_001727 --> ing23__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing23__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001727 --> ing24_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
ing25_Na_EDTA["Na-EDTA"]
CultureMech_001727 --> ing25_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing25_Na_EDTA -.-> CHEBI_64734
ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001727 --> ing26_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing27_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001727 --> ing27_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing27_MnSO4_x_H2O -.-> CHEBI_86364
ing28_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001727 --> ing28_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing28_CuSO4_x_5_H2O -.-> CHEBI_31440
ing29_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_001727 --> ing29_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing29_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
CultureMech_001727 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
50 G_PER_L |
CHEBI:9754
tris
|
|
| 4 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 5 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 6 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 7 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 8 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 9 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 10 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.009 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
23.47 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.92 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 13 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10.64 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 14 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.192 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 16 |
KCl
Cl.K 74.551 g/mol
|
0.664 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 17 |
KBr
Br.K 119.002 g/mol
|
0.096 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.026 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.024 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 20 |
NaF
F.Na 41.988 g/mol
|
0.003 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 21 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 22 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 23 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.335 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 24 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.00925 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 25 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.599 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.099, 0.5] |
| 26 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 27 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 28 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.154 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 29 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0392 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 30 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.0248 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
|
| 31 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Preparation
- Filter-sterilized.
- Dissolve the nitrilotriacetic acid.
- Adjust pH to 7.0 with KOH (~7.3 g).
- Dissolve other salts separately and combine.
- Adjust pH to 6.8 with NaOH or H2SO4.
- Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.685701Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 600 |
| 2026-03-13T03:08:41.868729Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:06.738844Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001727 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.955360+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M14 (PIRELLULA MARINA) MEDIUM" to "m14_pirellula_marina_medium" for consistent matching |
| 2026-06-05T07:41:11.698162+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.663134+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |