ancalomicrobium_medium

CultureMech:001731 bacterial DEFINED SOLID_AGAR
Original name: ANCALOMICROBIUM MEDIUM

Overview

External ontology
mediadive.medium:603 (source link unavailable) — ANCALOMICROBIUM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium603.pdf

Composition

flowchart LR
    CultureMech_001731["ancalomicrobium_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001731 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_Glucose["Glucose"]
    CultureMech_001731 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_001731 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_Agar["Agar"]
    CultureMech_001731 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Vitamin_B12["Vitamin B12"]
    CultureMech_001731 --> ing4_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing4_Vitamin_B12 -.-> CHEBI_176843
    ing5_Biotin["Biotin"]
    CultureMech_001731 --> ing5_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing5_Biotin -.-> CHEBI_15956
    ing6_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001731 --> ing6_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing6_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing7_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001731 --> ing7_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing7_Calcium_pantothenate -.-> CHEBI_31345
    ing8_Folic_acid["Folic acid"]
    CultureMech_001731 --> ing8_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing8_Folic_acid -.-> CHEBI_27470
    ing9_Riboflavin["Riboflavin"]
    CultureMech_001731 --> ing9_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing9_Riboflavin -.-> CHEBI_17015
    ing10_Nicotinamide["Nicotinamide"]
    CultureMech_001731 --> ing10_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing10_Nicotinamide -.-> CHEBI_17154
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001731 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001731 --> ing12_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001731 --> ing13_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing14__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001731 --> ing14__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing14__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001731 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_Na_EDTA["Na-EDTA"]
    CultureMech_001731 --> ing16_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing16_Na_EDTA -.-> CHEBI_64734
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001731 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001731 --> ing18_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing18_MnSO4_x_H2O -.-> CHEBI_86364
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001731 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001731 --> ing20_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing20_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing21_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001731 --> ing21_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing21_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.247525 G_PER_L CHEBI:62946
ammonium sulfate
2 Glucose
C6H12O6 180.156 g/mol
0.247525 G_PER_L CHEBI:17234
glucose
3 Na2HPO4
HO4P.2Na 141.958 g/mol
0.070297 G_PER_L CHEBI:34683
disodium hydrogenphosphate
4 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
5 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
6 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
7 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
8 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
9 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
10 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
11 Nicotinamide
C6H6N2O 122.127 g/mol
0.005 G_PER_L CHEBI:17154
nicotinamide
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
14 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
15 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
17 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
22 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. pH 7
  2. Make up the medium without the vitamin solution and autoclave it. The filter-sterilized vitamin solution is added to the cooled medium. The medium may be solidified by adding 15 g/l agar.
  3. Filter-sterilized
  4. Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
  5. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.704140Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 603
2026-03-13T03:08:56.830163Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:06.794182Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001731
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.017622+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ANCALOMICROBIUM MEDIUM" to "ancalomicrobium_medium" for consistent matching
2026-06-05T07:40:49.202759+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.350325+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.