nutrient_agar_oxoid_cm3_with_phosphate

CultureMech:001733 bacterial COMPLEX SOLID_AGAR
Original name: NUTRIENT AGAR (OXOID CM3) WITH PHOSPHATE

Overview

External ontology
mediadive.medium:605a (source link unavailable) — NUTRIENT AGAR (OXOID CM3) WITH PHOSPHATE
Applications
  • Microbial cultivation
pH
6.8
Notes
DSMZ Medium 605a supplements DSMZ Medium 605 with 0.45 g/L KH2PO4 and 2.39 g/L Na2HPO4 x 12 H2O; the final pH is 6.8.

Composition

flowchart LR
    CultureMech_001733["nutrient_agar_oxoid_cm3_with_phosphate"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001733 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_001733 --> ing1_Na2HPO4_x_12_H2O
    CHEBI_91259["CHEBI:91259"]
    ing1_Na2HPO4_x_12_H2O -.-> CHEBI_91259
    ing2_Lab_Lemco_beef_extract["Lab-Lemco beef extract"]
    CultureMech_001733 --> ing2_Lab_Lemco_beef_extract
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001733 --> ing3_Yeast_extract
    ing4_Peptone["Peptone"]
    CultureMech_001733 --> ing4_Peptone
    ing5_NaCl["NaCl"]
    CultureMech_001733 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_001733 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Distilled_water["Distilled water"]
    CultureMech_001733 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.45 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 DSMZ Medium 605a adds 0.45 g/L KH2PO4 to DSMZ Medium 605.
2 Na2HPO4 x 12 H2O
12H2O.HO4P.2Na 358.138 g/mol
2.39 G_PER_L CHEBI:91259
disodium hydrogenphosphate dodecahydrate
 DSMZ Medium 605a adds 2.39 g/L Na2HPO4 x 12 H2O to DSMZ Medium 605.
3 Lab-Lemco beef extract 1.0 G_PER_L FOODON:03302088
Beef extract
 DSMZ Medium 605 lists 1.0 g/L Lab-Lemco beef extract.
4 Yeast extract 2.0 G_PER_L FOODON:03315426
Yeast extract
 DSMZ Medium 605 lists 2.0 g/L Yeast extract.
5 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 DSMZ Medium 605 lists 5.0 g/L Peptone.
6 NaCl
Cl.Na 58.443 g/mol
5.0 G_PER_L CHEBI:26710
sodium chloride
 DSMZ Medium 605 lists 5.0 g/L NaCl.
7 Agar 15.0 G_PER_L CHEBI:2509
agar
 DSMZ Medium 605 lists 15.0 g/L Agar.
8 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 DSMZ Medium 605 lists 1000.0 ml Distilled water.

Preparation

  1. Add 0.45 g/L KH2PO4 and 2.39 g/L Na2HPO4 x 12 H2O to DSMZ Medium 605; final pH is 6.8.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.709359Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 605a
2026-03-13T06:19:06.803316Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001733
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.027928+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NUTRIENT AGAR (OXOID CM3) WITH PHOSPHATE" to "nutrient_agar_oxoid_cm3_with_phosphate" for consistent matching
2026-06-05T07:41:40.135646+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.700608+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00 repair_nutrient_ncg_nssm_score15.py RESOLVED_DSMZ_605A_SCORE15 DSMZ Medium 605a supplements DSMZ Medium 605 with 0.45 g/L KH2PO4 and 2.39 g/L Na2HPO4 x 12 H2O; the final pH is 6.8.