colby_and_zathman_medium

CultureMech:001734 bacterial DEFINED SOLID_AGAR
Original name: COLBY AND ZATHMAN MEDIUM

Overview

External ontology
mediadive.medium:606 (source link unavailable) — COLBY AND ZATHMAN MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium606.pdf

Composition

flowchart LR
    CultureMech_001734["colby_and_zathman_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001734 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001734 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_001734 --> ing2_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing2_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001734 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_NaCl["NaCl"]
    CultureMech_001734 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001734 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_Agar["Agar"]
    CultureMech_001734 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Methanol["Methanol"]
    CultureMech_001734 --> ing7_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing7_Methanol -.-> CHEBI_17790
    ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001734 --> ing8_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing9_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
    CultureMech_001734 --> ing9_MnSO4_x_5_H2O
    CHEBI_131524["CHEBI:131524"]
    ing9_MnSO4_x_5_H2O -.-> CHEBI_131524
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001734 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_H3BO3["H3BO3"]
    CultureMech_001734 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001734 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001734 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001734 --> ing14_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing14_FeCl3_x_6_H2O -.-> CHEBI_86254

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
1.2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.62 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.05 G_PER_L CHEBI:91243
calcium chloride hexahydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 NaCl
Cl.Na 58.443 g/mol
0.1 G_PER_L CHEBI:26710
sodium chloride
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
7 Agar 15 G_PER_L CHEBI:2509
agar
8 Methanol
CH4O 32.042 g/mol
1.584 G_PER_L CHEBI:17790
methanol
9 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.005 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
10 MnSO4 x 5 H2O
5H2O.Mn.O4S 241.076 g/mol
0.01 G_PER_L CHEBI:131524
manganese(II) sulfate pentahydrate
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
12 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.07 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.005 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1 G_PER_L CHEBI:86254
iron trichloride hexahydrate

Preparation

  1. Adjust pH to 7.0. Autoclave at 121°C for 15 min. Cool to 50°C. Add a filter-sterilized solution of methanol to give a final concentration of 0.2%.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.710743Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 606
2026-03-13T06:19:06.809554Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001734
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.035050+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "COLBY AND ZATHMAN MEDIUM" to "colby_and_zathman_medium" for consistent matching
2026-06-05T07:40:54.601336+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.931760+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.