Overview
- External ontology
- mediadive.medium:60 (source link unavailable) — MOORELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.9
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium60.pdf
Composition
flowchart LR
CultureMech_001738["moorella_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001738 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_001738 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_Fe_NH4_2_SO4_2["Fe(NH4)2(SO4)2"]
CultureMech_001738 --> ing2_Fe_NH4_2_SO4_2
ing3_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001738 --> ing3_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing3_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing4_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001738 --> ing4_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing4_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing5_Tryptone["Tryptone"]
CultureMech_001738 --> ing5_Tryptone
ing6_Yeast_extract["Yeast extract"]
CultureMech_001738 --> ing6_Yeast_extract
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001738 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_K2HPO4["K2HPO4"]
CultureMech_001738 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_KH2PO4["KH2PO4"]
CultureMech_001738 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10_Glucose["Glucose"]
CultureMech_001738 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11_Na2CO3["Na2CO3"]
CultureMech_001738 --> ing11_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3 -.-> CHEBI_29377
ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001738 --> ing12_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001738 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.118624 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.59312 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 | Fe(NH4)2(SO4)2 | 0.0474496 G_PER_L | ||
| 4 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.00284698 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 5 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.000177936 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 6 | Tryptone | 5.9312 G_PER_L | ||
| 7 | Yeast extract | 5.9312 G_PER_L | ||
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00059312 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
140 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
90 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
360 G_PER_L |
CHEBI:17234
glucose
|
|
| 12 |
Na2CO3
CO3.2Na 105.988 g/mol
|
50 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 13 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
30 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
30 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Sparge solution A with 100% CO2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Autoclave separately solutions B, C, E and F under 100% N2 gas, and solution D under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.9, if necessary.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.067170Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 60 |
| 2026-03-13T06:19:06.849167Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001738 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.077777+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MOORELLA MEDIUM" to "moorella_medium" for consistent matching |
| 2026-06-05T07:41:38.183828+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.091429+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |