Overview
- External ontology
- mediadive.medium:615 (source link unavailable) — DESULFOVIBRIO (MG-1) MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium615.pdf
Composition
flowchart LR
CultureMech_001744["desulfovibrio_mg_1_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001744 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_001744 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Na2SO4["Na2SO4"]
CultureMech_001744 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001744 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001744 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Glycerol["Glycerol"]
CultureMech_001744 --> ing5_Glycerol
CHEBI_17754["CHEBI:17754"]
ing5_Glycerol -.-> CHEBI_17754
ing6_Yeast_extract["Yeast extract"]
CultureMech_001744 --> ing6_Yeast_extract
ing7_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_001744 --> ing7_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing7_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_001744 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001744 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_Na_thioglycolate["Na-thioglycolate"]
CultureMech_001744 --> ing10_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing10_Na_thioglycolate -.-> CHEBI_86481
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.996016 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.498008 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4.48207 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0398406 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.059761 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
Glycerol
C3H8O3 92.094 g/mol
|
1.99203 G_PER_L |
CHEBI:17754
glycerol
|
|
| 7 | Yeast extract | 0.996016 G_PER_L | ||
| 8 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
0.59761 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000498008 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00398406 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.0996016 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Preparation
- Dissolve ingredients, adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.743785Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 615 |
| 2026-01-28T19:48:33.996985Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:06.916683Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001744 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.151038+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOVIBRIO (MG-1) MEDIUM" to "desulfovibrio_mg_1_medium" for consistent matching |
| 2026-06-05T07:40:57.928076+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.085868+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |