hyphomicrobium_methylovorum_medium

CultureMech:001749 bacterial DEFINED SOLID_AGAR
Original name: HYPHOMICROBIUM METHYLOVORUM MEDIUM

Overview

External ontology
mediadive.medium:619 (source link unavailable) — HYPHOMICROBIUM METHYLOVORUM MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium619.pdf

Composition

flowchart LR
    CultureMech_001749["hyphomicrobium_methylovorum_medium"]
    ing0_Methanol["Methanol"]
    CultureMech_001749 --> ing0_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing0_Methanol -.-> CHEBI_17790
    ing1__NH4_2HPO4["(NH4)2HPO4"]
    CultureMech_001749 --> ing1__NH4_2HPO4
    CHEBI_63051["CHEBI:63051"]
    ing1__NH4_2HPO4 -.-> CHEBI_63051
    ing2_NaCl["NaCl"]
    CultureMech_001749 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001749 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001749 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_MnSO4_x_1_H2O["MnSO4 x 1 H2O"]
    CultureMech_001749 --> ing5_MnSO4_x_1_H2O
    CHEBI_86360["CHEBI:86360"]
    ing5_MnSO4_x_1_H2O -.-> CHEBI_86360
    ing6_Agar["Agar"]
    CultureMech_001749 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Tap_water["Tap water"]
    CultureMech_001749 --> ing7_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Tap_water -.-> CHEBI_15377
    ing8_Biotin["Biotin"]
    CultureMech_001749 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001749 --> ing9_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing9_Calcium_pantothenate -.-> CHEBI_31345
    ing10_Folic_acid["Folic acid"]
    CultureMech_001749 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Inositol["Inositol"]
    CultureMech_001749 --> ing11_Inositol
    CHEBI_24848["CHEBI:24848"]
    ing11_Inositol -.-> CHEBI_24848
    ing12_Niacin["Niacin"]
    CultureMech_001749 --> ing12_Niacin
    CHEBI_15940["CHEBI:15940"]
    ing12_Niacin -.-> CHEBI_15940
    ing13_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001749 --> ing13_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing13_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001749 --> ing14_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing15_Riboflavin["Riboflavin"]
    CultureMech_001749 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001749 --> ing16_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing16_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing17_Choline["Choline"]
    CultureMech_001749 --> ing17_Choline
    CHEBI_15354["CHEBI:15354"]
    ing17_Choline -.-> CHEBI_15354
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_001749 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
2 (NH4)2HPO4
2H4N.HO4P 132.056 g/mol
3 G_PER_L CHEBI:63051
diammonium hydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
6 MnSO4 x 1 H2O
Mn.O4S 151.001 g/mol
0.005 G_PER_L CHEBI:86360
manganese(II) sulfate
7 Agar 15 G_PER_L CHEBI:2509
agar
8 Tap water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
9 Biotin
C10H16N2O3S 244.316 g/mol
0.0002 G_PER_L CHEBI:15956
biotin
10 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.04 G_PER_L CHEBI:31345
Calcium pantothenate
11 Folic acid
C19H19N7O6 441.404 g/mol
0.0002 G_PER_L CHEBI:27470
folic acid
12 Inositol
C6H12O6 180.156 g/mol
0.2 G_PER_L CHEBI:24848
inositol
13 Niacin
C6H5NO2 123.111 g/mol
0.04 G_PER_L CHEBI:15940
nicotinic acid
14 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.02 G_PER_L CHEBI:30753
4-aminobenzoic acid
15 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.04 G_PER_L CHEBI:30961
pyridoxine hydrochloride
16 Riboflavin
C17H20N4O6 376.369 g/mol
0.04 G_PER_L CHEBI:17015
riboflavin
17 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.02 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
18 Choline
C5H14NO 104.173 g/mol
0.1 G_PER_L CHEBI:15354
choline
19 Vitamin B12 2e-06 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Sterilize methanol by filtration, and add to other components sterilized by autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.751495Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 619
2026-03-13T06:19:06.939194Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001749
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.178018+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HYPHOMICROBIUM METHYLOVORUM MEDIUM" to "hyphomicrobium_methylovorum_medium" for consistent matching
2026-06-05T07:41:09.222517+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.991818+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:59.993964+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.