ekho_lake_strains_medium

CultureMech:001753 bacterial COMPLEX SOLID_AGAR
Original name: EKHO LAKE STRAINS MEDIUM

Overview

External ontology
mediadive.medium:621a (source link unavailable) — EKHO LAKE STRAINS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium621a.pdf

Composition

flowchart LR
    CultureMech_001753["ekho_lake_strains_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_001753 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001753 --> ing1_Yeast_extract
    ing2_Agar["Agar"]
    CultureMech_001753 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Glucose["Glucose"]
    CultureMech_001753 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_NaCl["NaCl"]
    CultureMech_001753 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Na2SO4["Na2SO4"]
    CultureMech_001753 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001753 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_CaCl2["CaCl2"]
    CultureMech_001753 --> ing7_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing7_CaCl2 -.-> CHEBI_3312
    ing8_NaHCO3["NaHCO3"]
    CultureMech_001753 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_KCl["KCl"]
    CultureMech_001753 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_KBr["KBr"]
    CultureMech_001753 --> ing10_KBr
    CHEBI_32030["CHEBI:32030"]
    ing10_KBr -.-> CHEBI_32030
    ing11_H3BO3["H3BO3"]
    CultureMech_001753 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_SrCl2["SrCl2"]
    CultureMech_001753 --> ing12_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing12_SrCl2 -.-> CHEBI_36383
    ing13_NaF["NaF"]
    CultureMech_001753 --> ing13_NaF
    CHEBI_28741["CHEBI:28741"]
    ing13_NaF -.-> CHEBI_28741
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001753 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001753 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001753 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001753 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001753 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_Biotin["Biotin"]
    CultureMech_001753 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_001753 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001753 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Riboflavine["Riboflavine"]
    CultureMech_001753 --> ing22_Riboflavine
    CHEBI_17015["CHEBI:17015"]
    ing22_Riboflavine -.-> CHEBI_17015
    ing23_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001753 --> ing23_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing23_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing24_Nicotinamide["Nicotinamide"]
    CultureMech_001753 --> ing24_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing24_Nicotinamide -.-> CHEBI_17154
    ing25_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_001753 --> ing25_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_D_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_001753 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001753 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 Peptone 0.25 G_PER_L
2 Yeast extract 0.25 G_PER_L
3 Agar 15 G_PER_L CHEBI:2509
agar
4 Glucose
C6H12O6 180.156 g/mol
0.25 G_PER_L CHEBI:17234
glucose
5 NaCl
Cl.Na 58.443 g/mol
23.477 G_PER_L CHEBI:26710
sodium chloride
6 Na2SO4
2Na.O4S 142.043 g/mol
3.917 G_PER_L CHEBI:32149
sodium sulfate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.981 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 CaCl2
Ca.2Cl 110.984 g/mol
1.102 G_PER_L CHEBI:3312
calcium dichloride
9 NaHCO3
CHO3.Na 84.006 g/mol
0.192 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 KCl
Cl.K 74.551 g/mol
0.664 G_PER_L CHEBI:32588
potassium chloride
11 KBr
Br.K 119.002 g/mol
0.006 G_PER_L CHEBI:32030
potassium bromide
12 H3BO3
H3BO3 61.833 g/mol
0.026 G_PER_L CHEBI:33118
boric acid
13 SrCl2
2Cl.Sr 158.526 g/mol
0.024 G_PER_L CHEBI:36383
strontium dichloride
14 NaF
F.Na 41.988 g/mol
0.003 G_PER_L CHEBI:28741
sodium fluoride
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10.5263 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
31.2632 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.51579 G_PER_L CHEBI:86158
calcium chloride dihydrate
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0133368 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.104211 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
21 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
23 Riboflavine
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
24 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
25 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
26 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
27 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid

Preparation

  1. Sterilize 20 min./121°C. After cooling to 60°C add to the medium:
  2. The percentage Artificial Sea Water (ASW) indicates the percentage ratio of distilled water to artifical sea water (i.e. "medium 621a + 25ASW" = medium 621 made with 750 ml distilled water + 250 ml ASW). The pH is 7.2 - 7.4.
  3. Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.
  4. Store in the dark and cold (5°C).

Solutions

Metall salt sol. 44

Dose: 50 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.758876Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 621a
2026-01-28T19:48:23.431947Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:38.952053+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:06.969902Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001753
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.213426+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "EKHO LAKE STRAINS MEDIUM" to "ekho_lake_strains_medium" for consistent matching
2026-05-16T09:03:31.386923+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-05-20T06:01:16.149150+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4}
2026-06-05T07:40:59.461966+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.671008+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.