thermococcus_litoralis_medium

CultureMech:001755 archaea COMPLEX LIQUID
Original name: THERMOCOCCUS LITORALIS MEDIUM

Overview

External ontology
mediadive.medium:623 (source link unavailable) — THERMOCOCCUS LITORALIS MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium623.pdf

Composition

flowchart LR
    CultureMech_001755["thermococcus_litoralis_medium"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_001755 --> ing0_Bacto_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001755 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_001755 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001755 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_Na2SO4["Na2SO4"]
    CultureMech_001755 --> ing4_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing4_Na2SO4 -.-> CHEBI_32149
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001755 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KCl["KCl"]
    CultureMech_001755 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Na2CO3["Na2CO3"]
    CultureMech_001755 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_KBr["KBr"]
    CultureMech_001755 --> ing8_KBr
    CHEBI_32030["CHEBI:32030"]
    ing8_KBr -.-> CHEBI_32030
    ing9_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001755 --> ing9_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing9_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing10_Boric_acid["Boric acid"]
    CultureMech_001755 --> ing10_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing10_Boric_acid -.-> CHEBI_33118
    ing11_Na_meta_silicate["Na-meta silicate"]
    CultureMech_001755 --> ing11_Na_meta_silicate
    ing12_NaF["NaF"]
    CultureMech_001755 --> ing12_NaF
    CHEBI_28741["CHEBI:28741"]
    ing12_NaF -.-> CHEBI_28741
    ing13_KNO3["KNO3"]
    CultureMech_001755 --> ing13_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing13_KNO3 -.-> CHEBI_63043
    ing14_Na2HPO4["Na2HPO4"]
    CultureMech_001755 --> ing14_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing14_Na2HPO4 -.-> CHEBI_34683
    ing15_Sulfur["Sulfur"]
    CultureMech_001755 --> ing15_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing15_Sulfur -.-> CHEBI_26833
    ing16_Resazurin["Resazurin"]
    CultureMech_001755 --> ing16_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing16_Resazurin -.-> CHEBI_8806

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Bacto peptone 5 G_PER_L
2 Yeast extract 1 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
19.45 G_PER_L CHEBI:26710
sodium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
12.6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 Na2SO4
2Na.O4S 142.043 g/mol
3.42 G_PER_L CHEBI:32149
sodium sulfate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.38 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 KCl
Cl.K 74.551 g/mol
0.55 G_PER_L CHEBI:32588
potassium chloride
8 Na2CO3
CO3.2Na 105.988 g/mol
0.61 G_PER_L CHEBI:29377
sodium carbonate
9 KBr
Br.K 119.002 g/mol
0.08 G_PER_L CHEBI:32030
potassium bromide
10 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.057 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
11 Boric acid
H3BO3 61.833 g/mol
0.022 G_PER_L CHEBI:33118
boric acid
12 Na-meta silicate 0.004 G_PER_L
13 NaF
F.Na 41.988 g/mol
0.0024 G_PER_L CHEBI:28741
sodium fluoride
14 KNO3
K.NO3 101.102 g/mol
0.0016 G_PER_L CHEBI:63043
potassium nitrate
15 Na2HPO4
HO4P.2Na 141.958 g/mol
0.01 G_PER_L CHEBI:34683
disodium hydrogenphosphate
16 Sulfur
S 32.067 g/mol
10 G_PER_L CHEBI:26833
sulfur atom
17 Resazurin
C12H7NO4 229.191 g/mol
0.1 G_PER_L CHEBI:8806
Resazurin

Preparation

  1. Prepare the medium under 100% N2 and adjust final pH to 6.5. The medium, containing sulphur, is sterilized by steaming on three consecutive days for 3 h. Reduce the medium with a sterile Na2S stock solution to give a final concentration of 0.5 g/l.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.765039Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 623
2026-03-13T06:19:06.985959Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001755
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.233346+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOCOCCUS LITORALIS MEDIUM" to "thermococcus_litoralis_medium" for consistent matching
2026-06-05T07:41:49.903272+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:36.021611+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:12.911418+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.