Overview
- External ontology
- mediadive.medium:627 (source link unavailable) — SEAWATER LEMCO
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium627.pdf
Composition
flowchart LR
CultureMech_001758["seawater_lemco"]
ing0_Beef_extract["Beef extract"]
CultureMech_001758 --> ing0_Beef_extract
ing1_Peptone["Peptone"]
CultureMech_001758 --> ing1_Peptone
ing2_Sea_water["Sea water"]
CultureMech_001758 --> ing2_Sea_water
ing3_Agar["Agar"]
CultureMech_001758 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Beef extract | 10 G_PER_L |
FOODON:03302088
Beef extract
|
|
| 2 | Peptone | 10 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
|
| 3 | Sea water | 750 G_PER_L | ||
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Dissolve ingredients, by heating if necessary. Adjust pH to 7.8, boil 3-5 min, filter. Readjust pH to 7.3. Sterilize by autoclaving for 15 min at 121°C.
- For solid medium add 15.0 g agar after readjusting pH and steam to dissolve the agar prior to autoclaving.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.771823Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 627 |
| 2026-03-13T06:19:06.998518Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001758 |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.248662+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SEAWATER LEMCO" to "seawater_lemco" for consistent matching |
| 2026-06-05T07:41:45.473692+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch7.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH7 | Applied local mappings for Beef extract, Peptone. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |