acetobacter_europaeus_medium

Overview

External ontology

mediadive.medium:631 (source link unavailable) — ACETOBACTER EUROPAEUS MEDIUM

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium631.pdf

Composition

flowchart LR
    CultureMech_001762["acetobacter_europaeus_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001762 --> ing0_Yeast_extract
    ing1_Peptone["Peptone"]
    CultureMech_001762 --> ing1_Peptone
    ing2_Glucose["Glucose"]
    CultureMech_001762 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Acetic_acid["Acetic acid"]
    CultureMech_001762 --> ing3_Acetic_acid
    CHEBI_15366["CHEBI:15366"]
    ing3_Acetic_acid -.-> CHEBI_15366
    ing4_Ethanol["Ethanol"]
    CultureMech_001762 --> ing4_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing4_Ethanol -.-> CHEBI_16236

Ingredients (5)

Preparation

1. The ethanol and acetic acid are sterilized separately by filtration (use filter resistant to these solvents), and added aseptically to the sterile medium. Acetobacter europaeus will not grow on plates containing 1-2% agar, and a double layer system has to be used. A layer of growth medium containing 0.5% agar (about 35 ml) is poured into a plate and allowed to solidify. A thin layer (5-10 ml) of the growth medium containing 1% agar is then poured on top. Carefully streak the plates, which are best incubated in closed containers (e.g. plastic boxes) at 30°C.

Target organisms

No target organisms are recorded.

Provenance