nms_medium

CultureMech:001763 bacterial DEFINED SOLID_AGAR
Original name: NMS MEDIUM

Overview

External ontology
mediadive.medium:632 (source link unavailable) — NMS MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium632.pdf Cross-reference: ATCC:1306 (Nitrate Mineral Salts Medium (NMS))

Composition

flowchart LR
    CultureMech_001763["nms_medium"]
    ing0_Methanol["Methanol"]
    CultureMech_001763 --> ing0_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing0_Methanol -.-> CHEBI_17790
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001763 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_001763 --> ing2_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing2_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing3_Fe_III_NH4_EDTA["Fe(III)NH4-EDTA"]
    CultureMech_001763 --> ing3_Fe_III_NH4_EDTA
    ing4_KNO3["KNO3"]
    CultureMech_001763 --> ing4_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing4_KNO3 -.-> CHEBI_63043
    ing5_KH2PO4["KH2PO4"]
    CultureMech_001763 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_001763 --> ing6_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing6_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing7_Agar["Agar"]
    CultureMech_001763 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Na2_EDTA["Na2-EDTA"]
    CultureMech_001763 --> ing8_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing8_Na2_EDTA -.-> CHEBI_64734
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001763 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001763 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001763 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_001763 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001763 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001763 --> ing14_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001763 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001763 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Methanol
CH4O 32.042 g/mol
0.792 G_PER_L CHEBI:17790
methanol
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.2 G_PER_L CHEBI:91243
calcium chloride hexahydrate
4 Fe(III)NH4-EDTA 0.004 G_PER_L
5 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
6 KH2PO4
H2O4P.K 136.084 g/mol
0.272 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
0.717 G_PER_L CHEBI:34683
disodium hydrogenphosphate
8 Agar 12.5 G_PER_L CHEBI:2509
agar
9 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.01 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.003 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.02 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.001 G_PER_L CHEBI:86158
calcium chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.002 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 6.8. Autoclave at 121°C for 15 min. Methanol is added from a sterile stock solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.783818Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 632
2026-02-20T07:33:34.000000Z atcc-crossref-backfill Added ATCC cross-reference ATCC:1306 verified equivalent (verified 2026-01-06)
2026-03-13T06:19:07.039256Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001763
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.295728+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NMS MEDIUM" to "nms_medium" for consistent matching
2026-06-05T07:41:40.008616+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.700375+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:09.657685+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.