isp_medium_2_with_5_nacl

CultureMech:001770 bacterial COMPLEX SOLID_AGAR
Original name: ISP MEDIUM 2 WITH 5% NaCl

Overview

External ontology
mediadive.medium:636 (source link unavailable) — ISP MEDIUM 2 WITH 5% NaCl
Applications
  • Microbial cultivation
pH range
{'min': 7.3, 'max': 7.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium636.pdf

Composition

flowchart LR
    CultureMech_001770["isp_medium_2_with_5_nacl"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001770 --> ing0_Yeast_extract
    ing1_Malt_extract["Malt extract"]
    CultureMech_001770 --> ing1_Malt_extract
    ing2_Dextrose["Dextrose"]
    CultureMech_001770 --> ing2_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing2_Dextrose -.-> CHEBI_17634
    ing3_Agar["Agar"]
    CultureMech_001770 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_NaCl["NaCl"]
    CultureMech_001770 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Yeast extract 4 G_PER_L
2 Malt extract 10 G_PER_L
3 Dextrose
C6H12O6 180.156 g/mol
4 G_PER_L CHEBI:17634
D-glucose
4 Agar 20 G_PER_L CHEBI:2509
agar
5 NaCl
Cl.Na 58.443 g/mol
50 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. pH 7.3 - 7.5

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.797652Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 636
2026-03-13T06:19:07.078147Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001770
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.339005+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ISP MEDIUM 2 WITH 5% NaCl" to "isp_medium_2_with_5_nacl" for consistent matching
2026-05-20T06:01:23.383999+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5}
2026-06-05T07:41:09.700290+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.