Overview
- External ontology
- mediadive.medium:639 (source link unavailable) — MEDIUM WITH THIAZOLE
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium639.pdf
Composition
flowchart LR
CultureMech_001772["medium_with_thiazole"]
ing0_Benzothiazole["Benzothiazole"]
CultureMech_001772 --> ing0_Benzothiazole
ing1_NaOH["NaOH"]
CultureMech_001772 --> ing1_NaOH
CHEBI_32145["CHEBI:32145"]
ing1_NaOH -.-> CHEBI_32145
ing2_KH2PO4["KH2PO4"]
CultureMech_001772 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001772 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001772 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_FeCl3["FeCl3"]
CultureMech_001772 --> ing5_FeCl3
CHEBI_30808["CHEBI:30808"]
ing5_FeCl3 -.-> CHEBI_30808
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Benzothiazole | 0.333333 G_PER_L | ||
| 2 |
NaOH
HO.Na 39.997 g/mol
|
9 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.12 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.2 G_PER_L |
CHEBI:30808
iron trichloride
|
Preparation
- Autoclave solutions A, B and C separately and combine after cooling 100 ml solution A, 5 ml solution B and 5 ml solution C.
- Dissolve 30.0 mg benzothiazole in 90 ml 10% NaOH, bring pH to 7.0 with concentrated HCl.
- Dissolve 300 mg KH2PO4 in 50 ml distilled water.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.802327Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 639 |
| 2026-03-13T06:19:07.089078Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001772 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.350483+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM WITH THIAZOLE" to "medium_with_thiazole" for consistent matching |
| 2026-06-05T07:41:30.406049+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.552937+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |