middlebrook_medium

Overview

External ontology

mediadive.medium:645 (source link unavailable) — MIDDLEBROOK MEDIUM

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Source-specific ingredient identities, quantity bases and preparation routes supersede the copied flattened amounts. Historical events are preserved. Chemical groundings are not comprehensively recertified; no taxa-media association is added.

Composition

flowchart LR
    CultureMech_001782["middlebrook_medium"]
    ing0_Bacto_Middlebrook_7H10_agar["Bacto Middlebrook 7H10 agar"]
    CultureMech_001782 --> ing0_Bacto_Middlebrook_7H10_agar
    ing1_Glycerol["Glycerol"]
    CultureMech_001782 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_Distilled_water["Distilled water"]
    CultureMech_001782 --> ing2_Distilled_water

Ingredients (3)

Preparation

1. Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly.

Solutions

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  From-scratch alternative to commercial 7H10

  Relationship

  SUBSTITUTED_COMPONENT_VARIANT

  Description

  Use only when the commercial medium is unavailable. This is an alternative preparation, not simultaneous inputs to the commercial route; final-volume equivalence is unresolved.

  Modifications

  • Alternative preparation input: (NH4)2SO4 0.5 g; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: KH2PO4 1.5 g; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: Na2HPO4 1.5 g; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: Sodium citrate 0.4 g; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: MgSO4 0.025 g; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: CaCl2 0.5 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: ZnSO4 1 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: CuSO4 1 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: L-Glutamic acid 0.5 g; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: Ferric ammonium citrate 0.04 g; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: Pyridoxine 1 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: Biotin 0.5 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: Malachite green 0.25 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: Agar 15 g; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • Alternative preparation input: Distilled water 1000 ml; not an additional input to the commercial preparation. No final-medium concentration is inferred.
  • The alternative table lists 1000 ml water, not the commercial route's 900 ml. Do not sum those water inputs or assert measured final volume. Its final pH at 25 C is 6.6 +/- 0.2; no titrant or titration dose is specified.

  Evidence

  • Reference

    https://mediadive.dsmz.de/rest/medium/645

    Supports

    SUPPORT

    Snippet

    { "recipe_order": 1, "compound": "(NH4)2SO4", "compound_id": 27, "amount": 0.5, "unit": "g", "g_l": 0.5, "mmol_l": 3.78387, "optional": 0 }

    Explanation

    variants[0].modifications: Alternative preparation input: (NH4)2SO4 0.5 g; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[0]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 2, "compound": "KH2PO4", "compound_id": 11, "amount": 1.5, "unit": "g", "g_l": 1.5, "mmol_l": 11.0225, "optional": 0 } variants[0].modifications: Alternative preparation input: KH2PO4 1.5 g; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[1]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 3, "compound": "Na2HPO4", "compound_id": 233, "amount": 1.5, "unit": "g", "g_l": 1.5, "mmol_l": 10.5664, "optional": 0 } variants[0].modifications: Alternative preparation input: Na2HPO4 1.5 g; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[2]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 4, "compound": "Sodium citrate", "compound_id": 792, "amount": 0.4, "unit": "g", "g_l": 0.4, "mmol_l": 1.54997, "optional": 0 } variants[0].modifications: Alternative preparation input: Sodium citrate 0.4 g; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[3]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 5, "compound": "MgSO4", "compound_id": 325, "amount": 0.025, "unit": "g", "g_l": 0.025, "mmol_l": 0.207693, "optional": 0 } variants[0].modifications: Alternative preparation input: MgSO4 0.025 g; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[4]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 6, "compound": "CaCl2", "compound_id": 81, "amount": 0.5, "unit": "mg", "g_l": 0.0005, "mmol_l": 0.00450532, "optional": 0 } variants[0].modifications: Alternative preparation input: CaCl2 0.5 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[5]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 7, "compound": "ZnSO4", "compound_id": 441, "amount": 1, "unit": "mg", "g_l": 0.001, "mmol_l": 0.00619579, "optional": 0 } variants[0].modifications: Alternative preparation input: ZnSO4 1 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[6]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 8, "compound": "CuSO4", "compound_id": 636, "amount": 1, "unit": "mg", "g_l": 0.001, "mmol_l": 0.00626527, "optional": 0 } variants[0].modifications: Alternative preparation input: CuSO4 1 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[7]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 9, "compound": "L-Glutamic acid", "compound_id": 374, "amount": 0.5, "unit": "g", "g_l": 0.5, "mmol_l": 3.39836, "optional": 0 } variants[0].modifications: Alternative preparation input: L-Glutamic acid 0.5 g; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[8]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 10, "compound": "Ferric ammonium citrate", "compound_id": 97, "amount": 0.04, "unit": "g", "g_l": 0.04, "optional": 0 } variants[0].modifications: Alternative preparation input: Ferric ammonium citrate 0.04 g; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[9]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 11, "compound": "Pyridoxine", "compound_id": 637, "amount": 1, "unit": "mg", "g_l": 0.001, "mmol_l": 0.00591086, "optional": 0 } variants[0].modifications: Alternative preparation input: Pyridoxine 1 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[10]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 12, "compound": "Biotin", "compound_id": 46, "amount": 0.5, "unit": "mg", "g_l": 0.0005, "mmol_l": 0.00204658, "optional": 0 } variants[0].modifications: Alternative preparation input: Biotin 0.5 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[11]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 13, "compound": "Malachite green", "compound_id": 407, "amount": 0.25, "unit": "mg", "g_l": 0.00025, "mmol_l": 0.000685119, "optional": 0 } variants[0].modifications: Alternative preparation input: Malachite green 0.25 mg; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[12]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 14, "compound": "Agar", "compound_id": 3, "attribute": "Bacto ", "amount": 15, "unit": "g", "g_l": 15, "mmol_l": 44.5991, "optional": 0 } variants[0].modifications: Alternative preparation input: Agar 15 g; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[13]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: { "recipe_order": 15, "compound": "Distilled water", "compound_id": 4, "amount": 1000, "unit": "ml", "optional": 0 } variants[0].modifications: Alternative preparation input: Distilled water 1000 ml; not an additional input to the commercial preparation. No final-medium concentration is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].recipe[14]; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318. Additional exact excerpt: "Final pH at 25\u00b0C 6.6 \u00b1 0.2." variants[0].modifications: Preserve pH at the alternative-table scope, not a heating instruction. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=data.solutions[1].steps[0].step; accessed=2026-10-09T00:07:51.968862+00:00; capture SHA256=5d209fb06ff092aec86241b9d51276f02a45bf1203e441c5cc042f5e4c1ce318.

  • Reference

    https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf

    Supports

    SUPPORT

    Snippet

    If you can´t order the commercial media then prepare the media in this way: Bacto Middlebrook 7H10 agar: (NH4)2SO4 0.500 g KH2PO4 1.500 g Na2HPO4 1.500 g Na-citrate 0.400 g MgSO4 0.025 g CaCl2 0.500 mg ZnSO4 1.000 mg CuSO4 1.000 mg L-Glutamic acid 0.500 g Ferric ammonium citrate 0.040 g Pyridoxine 1.000 mg Biotin 0.500 mg Malachite green 0.250 mg Bacto agar 15.000 g Distilled water 1000.000 ml Final pH at 25C 6.6 ± 0.2.

    Explanation

    variants[0].description: Archive the displaced flattened ingredient nodes, including chemical annotations, in append-only history. Only the conditional alternative carries these basal inputs; its table does not establish final-volume equivalence to the commercial route. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T00:07:53.271442+00:00; capture SHA256=8f4d6801aab892b40a988bba7e499d582097489fae3871d760fbabc22e8e1757.

Provenance