Overview
- External ontology
- mediadive.medium:645a (source link unavailable) — HEMIN MEDIUM FOR MYCOBACTERIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645a.pdf
Composition
flowchart LR
CultureMech_001783["hemin_medium_for_mycobacterium"]
ing0_Middlebrook_7H10_agar["Middlebrook 7H10 agar"]
CultureMech_001783 --> ing0_Middlebrook_7H10_agar
CHEBI_2509["CHEBI:2509"]
ing0_Middlebrook_7H10_agar -.-> CHEBI_2509
ing1_OADC_Enrichment["OADC Enrichment"]
CultureMech_001783 --> ing1_OADC_Enrichment
ing2_Haemin["Haemin"]
CultureMech_001783 --> ing2_Haemin
CHEBI_50385["CHEBI:50385"]
ing2_Haemin -.-> CHEBI_50385
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Middlebrook 7H10 agar | 900 G_PER_L |
CHEBI:2509
agar
|
|
| 2 | OADC Enrichment | 100 G_PER_L | ||
| 3 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
60 MICROMOLAR |
CHEBI:50385
hemin
|
Preparation
- Middlebrook 7H10 (Difco 0627) with OADC Enrichment (Difco 0722) with 60 micromoles hemin. Hemin must be solubilized with 1N NAOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.825347Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 645a |
| 2026-03-13T06:19:07.186995Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001783 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.460062+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HEMIN MEDIUM FOR MYCOBACTERIUM" to "hemin_medium_for_mycobacterium" for consistent matching |
| 2026-06-05T07:41:08.408425+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |