clostridium_grantii_medium

Overview

External ontology

mediadive.medium:648 (source link unavailable) — CLOSTRIDIUM GRANTII MEDIUM

Applications

• Microbial cultivation

pH

6.6

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium648.pdf

Composition

flowchart LR
    CultureMech_001787["clostridium_grantii_medium"]
    ing0_Marine_Broth["Marine Broth"]
    CultureMech_001787 --> ing0_Marine_Broth
    ing1_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001787 --> ing1_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing1_Sodium_resazurin -.-> CHEBI_8806
    ing2_Na2CO3["Na2CO3"]
    CultureMech_001787 --> ing2_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing2_Na2CO3 -.-> CHEBI_29377
    ing3_D_Glucose["D-Glucose"]
    CultureMech_001787 --> ing3_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing3_D_Glucose -.-> CHEBI_17634
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001787 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001787 --> ing5_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing5_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (6)

Preparation

1. Dissolve DIFCO Marine Broth powder and resazurin, bring the medium to a boil, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense the medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete the medium by adding glucose, cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be around 6.6.

Target organisms

No target organisms are recorded.

Provenance