centenum_medium

CultureMech:001789 bacterial COMPLEX LIQUID
Original name: CENTENUM MEDIUM

Overview

External ontology
mediadive.medium:650 (source link unavailable) — CENTENUM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium650.pdf Preparation follow-up: water volumes are batch inputs, not measured final volumes. Original chemical mappings, history and source links are retained, not independently revalidated. Preparation follows the cited source; no dose into another receiving medium is inferred.

Composition

flowchart LR
    CultureMech_001789["centenum_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001789 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_MgSO4["MgSO4"]
    CultureMech_001789 --> ing1_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing1_MgSO4 -.-> CHEBI_32599
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001789 --> ing2_Yeast_extract
    ing3_Vitamin_B12["Vitamin B12"]
    CultureMech_001789 --> ing3_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing3_Vitamin_B12 -.-> CHEBI_176843
    ing4_Tap_water["Tap water"]
    CultureMech_001789 --> ing4_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Tap_water -.-> CHEBI_15377
    ing5_Pyruvate["Pyruvate"]
    CultureMech_001789 --> ing5_Pyruvate
    CHEBI_15361["CHEBI:15361"]
    ing5_Pyruvate -.-> CHEBI_15361

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Source input: 1 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a concentration in a separately prepared base, a separate stock, or a measured final-medium volume.
2 MgSO4
Mg.O4S 120.368 g/mol
0.5 G_PER_L CHEBI:32599
magnesium sulfate
 Source input: 0.5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a concentration in a separately prepared base, a separate stock, or a measured final-medium volume.
3 Yeast extract 10 G_PER_L Source input: 10 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a concentration in a separately prepared base, a separate stock, or a measured final-medium volume.
4 Vitamin B12 2e-05 G_PER_L CHEBI:176843
vitamin B12
 Source input: 20 µg. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a concentration in a separately prepared base, a separate stock, or a measured final-medium volume.
5 Tap water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 1000 ml / 1000 = 1 L tap-water input per batch, not a mass concentration, percentage or measured final volume.
6 Pyruvate
C3H3O3 87.054 g/mol
20 MILLIMOLAR CHEBI:15361
pyruvate
 Source-explicit 20 mM pyruvate added after autoclaving; no molecular-weight conversion or unspecified stock dose inferred.

Preparation

  1. Adjust pH to 7.0.
  2. Boil under nitrogen gas and autoclave at 121°C for 15 min. Add 20 mM pyruvate after autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.841054Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 650
2026-03-13T06:19:07.236599Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001789
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.516999+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CENTENUM MEDIUM" to "centenum_medium" for consistent matching
2026-06-05T07:40:52.978985+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.436794+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.
2026-10-08T04:23:42.348042+00:00 codex CORRECTED_DSMZ650_752_762_MEDIUM_AND_SOLUTION_PREPARATIONS Corrected source-explicit amounts and preparation stages; original excerpts and history retained. Earlier carbonate concentration claims explicitly withdrawn and replaced by scoped input-mass evidence. No new growth claim or chemical grounding.