Overview
- External ontology
- mediadive.medium:654 (source link unavailable) — SPIRILLUM GRACILE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium654.pdf
Composition
flowchart LR
CultureMech_001792["spirillum_gracile_medium"]
ing0_Peptone["Peptone"]
CultureMech_001792 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001792 --> ing1_Yeast_extract
ing2_Tween_80["Tween 80"]
CultureMech_001792 --> ing2_Tween_80
CHEBI_53426["CHEBI:53426"]
ing2_Tween_80 -.-> CHEBI_53426
ing3_K2HPO4["K2HPO4"]
CultureMech_001792 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Agar["Agar"]
CultureMech_001792 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Tap_water["Tap water"]
CultureMech_001792 --> ing5_Tap_water
CHEBI_15377["CHEBI:15377"]
ing5_Tap_water -.-> CHEBI_15377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.02 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if required) |
| 6 |
Tap water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Preparation
- Adjust pH to 7.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.848954Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 654 |
| 2026-03-13T06:19:07.253464Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001792 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.536211+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIRILLUM GRACILE MEDIUM" to "spirillum_gracile_medium" for consistent matching |
| 2026-06-05T07:41:46.204835+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.589868+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |