Overview
- External ontology
- mediadive.medium:660 (source link unavailable) — ALCALIGENES MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium660.pdf
Composition
flowchart LR
CultureMech_001798["alcaligenes_medium"]
ing0_Tris["Tris"]
CultureMech_001798 --> ing0_Tris
CHEBI_9754["CHEBI:9754"]
ing0_Tris -.-> CHEBI_9754
ing1_NaCl["NaCl"]
CultureMech_001798 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KCl["KCl"]
CultureMech_001798 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_NH4Cl["NH4Cl"]
CultureMech_001798 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Na2SO4["Na2SO4"]
CultureMech_001798 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001798 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001798 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_001798 --> ing7_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing8_Fe_NH4_citrate["Fe(NH4)citrate"]
CultureMech_001798 --> ing8_Fe_NH4_citrate
CHEBI_31604["CHEBI:31604"]
ing8_Fe_NH4_citrate -.-> CHEBI_31604
ing9_CuSO4["CuSO4"]
CultureMech_001798 --> ing9_CuSO4
CHEBI_23414["CHEBI:23414"]
ing9_CuSO4 -.-> CHEBI_23414
ing10_Sodium_succinate["Sodium succinate"]
CultureMech_001798 --> ing10_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing10_Sodium_succinate -.-> CHEBI_63675
ing11_HCl["HCl"]
CultureMech_001798 --> ing11_HCl
CHEBI_17883["CHEBI:17883"]
ing11_HCl -.-> CHEBI_17883
ing12_ZnCl2["ZnCl2"]
CultureMech_001798 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001798 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_001798 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001798 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001798 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001798 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001798 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Tris
C4H11NO3 121.136 g/mol
|
6.06 G_PER_L |
CHEBI:9754
tris
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
4.68 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
1.49 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.07 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.43 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.03 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
0.23 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 9 | Fe(NH4)citrate | 0.005 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 10 |
CuSO4
Cu.O4S 159.609 g/mol
|
0.399025 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
|
| 11 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
4 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 12 |
HCl
HCl 36.461 g/mol
|
0.25 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.06 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.04 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- After autoclaving add 2.5 ml 1.0 M CuSO4 and 10 ml of a 40% Na-succinate solution, both sterilized by filtration.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.857927Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 660 |
| 2026-03-13T06:19:07.281689Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001798 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.568307+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALCALIGENES MEDIUM" to "alcaligenes_medium" for consistent matching |
| 2026-06-05T07:40:47.643664+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.712740+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |