Overview
- External ontology
- mediadive.medium:665 (source link unavailable) — SULFOBACILLUS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 1.9, 'max': 2.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium665.pdf
Composition
flowchart LR
CultureMech_001804["sulfobacillus_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_001804 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KCl["KCl"]
CultureMech_001804 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_K2HPO4["K2HPO4"]
CultureMech_001804 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001804 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Ca_NO3_2["Ca(NO3)2"]
CultureMech_001804 --> ing4_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing4_Ca_NO3_2 -.-> CHEBI_64205
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001804 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_H2SO4["H2SO4"]
CultureMech_001804 --> ing6_H2SO4
CHEBI_26836["CHEBI:26836"]
ing6_H2SO4 -.-> CHEBI_26836
ing7_Yeast_extract["Yeast extract"]
CultureMech_001804 --> ing7_Yeast_extract
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
4.28571 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.142857 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.714286 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.714286 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
0.0142857 G_PER_L |
CHEBI:64205
calcium nitrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
146.844 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
H2SO4
H2O4S 98.079 g/mol
|
1 G_PER_L |
CHEBI:26836
sulfuric acid
|
(10 N) |
| 8 | Yeast extract | 0.2 G_PER_L |
Preparation
- After autoclaving, combine the three solutions. Medium pH 1.9 - 2.4.
- Adjust pH to 2.0 - 2.2 with sulfuric acid.
- (sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron)
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.875640Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 665 |
| 2026-02-06T05:10:14.799006Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:07.335537Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001804 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.631456+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFOBACILLUS MEDIUM" to "sulfobacillus_medium" for consistent matching |
| 2026-05-20T06:02:06.376499+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '1.9-2.4' -> {'min': 1.9, 'max': 2.4} |
| 2026-06-05T07:41:47.381887+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.679296+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:11.991489+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |